▎ 摘　　要

NOVELTY - Preparation of fluorescence resonance energy transfer sensor and detection of cauliflower mosaic virus 35S involves preparing graphene quantum dot solution, silver nanoparticle dispersion and graphene quantum dot dispersion-probe 1, adding 1-(3 propyl)-3 carbodiimide hydrochloride solution to graphene quantum dot solution, oscillating, adding N-hydroxyl succinimide solution, shaking, adding graphene quantum dot dispersion-probe 1 stock solution, reacting, washing with ethanol, centrifuging, and dispersing in tris-hydrochloric acid buffer solution. USE - Method for preparing fluorescence resonance energy transfer sensor and detecting cauliflower mosaic virus 35S. ADVANTAGE - The sensor has high specificity and is not easily modified. DETAILED DESCRIPTION - Preparation of fluorescence resonance energy transfer sensor and detection of cauliflower mosaic virus 35S comprises preparing graphene quantum dot solution, silver nanoparticle dispersion and graphene quantum dot dispersion-probe 1, adding 1-(3 propyl)-3 carbodiimide hydrochloride solution to graphene quantum dot solution, oscillating, adding N-hydroxyl succinimide solution, shaking, adding graphene quantum dot dispersion-probe 1 stock solution, reacting, washing with ethanol, centrifuging, dispersing in tris-hydrochloric acid buffer solution, reacting silver nanoparticle dispersion and stock solution, adding tris-hydrochloric acid buffer solution and sodium chloride solution, oscillating, reacting, washing, centrifuging, dispersing in tris-hydrochloric acid buffer solution, constructing fluorescence resonance energy transfer sensor, dispersing silver dispersion, adding sodium chloride solution, detecting cauliflower mosaic virus 35S on target DNA solution, establishing standard curve, incubating, adding sodium chloride solution, incubating, measuring fluorescent intensity at 355 nm, establishing standard curve, and determining fluorescence quenching efficiency.