▎ 摘　　要

NOVELTY - Colorimetric method for detecting dopamine involves preparing graphene oxide-platinum-copper nanoparticles by adding 7.6 mL ultrapure water and 0.4 mL graphene oxide solution into a reaction flask, adding 124 mu L chloroplatinic acid and 40 mu L copper chloride to the above solution, stirring, adding 500 mu L sodium borohydride solution to the flask, stirring, adding 100 mu L of graphene oxide-platinum-copper nano-enzyme mimic and 100 mu L dopamine in 0.6 mL phosphate buffer and reacting at 60 degrees C, incubating the obtained mixture at 60 degrees C, and recording the absorbance of the mixture at 440 nm. USE - Colorimetric method for detecting dopamine used for nanoscience, analytical chemistry, medical, and biomedical applications. ADVANTAGE - The graphene oxide-platinum-copper nanohybrid enzyme mimic used in the method has improved stability in harsh environments and flexibility of structure with low difficulty of modification. The graphene oxide-platinum-copper nanohybrid enzyme mimic is convenient to synthetize. DETAILED DESCRIPTION - Colorimetric method for detecting dopamine using graphene oxide-platinum-copper nanohybrid enzyme mimic, involves preparing graphene oxide-platinum-copper nanoparticles by adding 7.6 mL ultrapure water and 0.4 mL graphene oxide solution into a reaction flask, adding 124 mu L chloroplatinic acid and 40 mu L copper chloride to the above solution, stirring the obtained mixture in an ice bath for 5 minutes, preparing 0.4 mg/mL sodium borohydride solution and adding 500 mu L of the prepared solution to the reaction flask at a speed of 10 seconds/drop, stirring the obtained solution in the ice bath for another 1 hour after completion of addition of sodium borohydride solution, adding 100 mu L of graphene oxide-platinum-copper nano-enzyme mimic and 100 mu L dopamine in 0.6 mL phosphate buffer (pH 3.0) and reacting at 60 degrees C, incubating the obtained mixture at 60 degrees C for 120 minutes, and recording the absorbance of the mixture at 440 nm.