• 专利标题:   Cell impedance electrode, preferably single cell impedance electrode or double cell impedance electrode with three-dimensional graphene interface, comprises electrode base and capture groove which is provided on electrode base.
  • 专利号:   CN107631977-A
  • 发明人:   AIPING L, BENYONG C, XIAHUA W, HUAPING W, YUN X, WEIZHONG X, QI Z
  • 专利权人:   UNIV ZHEJIANG SCI TECH
  • 国际专利分类:   G01N015/10
  • 专利详细信息:   CN107631977-A 26 Jan 2018 G01N-015/10 201819 Pages: 28 Chinese
  • 申请详细信息:   CN107631977-A CN10670087 08 Aug 2017
  • 优先权号:   CN10670087

▎ 摘  要

NOVELTY - A cell impedance electrode, preferably single cell impedance electrode or double cell impedance electrode with three-dimensional graphene interface comprises electrode base and capture groove which is provided on the electrode base, where the capture groove comprises several microelectrodes, a gap of 5 mu m is provided between adjacent microelectrodes and several microelectrodes are arranged in an orderly manner to form an arc-like capture surface perpendicular to the electrode base. USE - Cell impedance electrode, preferably single cell impedance electrode or double cell impedance electrode. ADVANTAGE - The cell impedance electrode has high sensitivity and cell sensing performance, attains real-time quantitative collection of micromotion of single/double cells, such as cell adsorption, cell migration, cell proliferation and apoptosis, attains differentiation and case detection of single/double cancer cells at different stages of canceration and judges different physiological behaviors of cancer cells, such as capture, adhesion, migration, proliferation, differentiation and apoptosis. DETAILED DESCRIPTION - A cell impedance electrode, preferably single cell impedance electrode or double cell impedance electrode with three-dimensional graphene interface comprises electrode base and capture groove which is provided on the electrode base, where the capture groove comprises several microelectrodes, a gap of 5 mu m is provided between adjacent microelectrodes, several microelectrodes are arranged in an orderly manner to form an arc-like capture surface perpendicular to the electrode base, the arc is a semi-ellipse divided along minor axis, in case of single cell impedance electrode, the arc is a semi-elliptical arc divided along minor axis, in case of double cell impedance electrode, minor axis length is 16-20 um, in case of single cell impedance electrode, minor axis length is 27-33 um, in case of double cell impedance electrode, major axis length is 32-36 um, in case of single cell impedance electrode and major axis length is 40-45 um, in case of double cell impedance electrode.