▎ 摘　　要

NOVELTY - Preparing three-dimensional graphene nano-composite material comprises e.g. performing the ultrasonic processing graphene oxide in de-ionized water for 0.5-1 hours to obtain the graphene oxide dispersion, placing in a container, adding 70-90 mg L-cysteine dissolving in the graphene oxide dispersion liquid, and dripping 400-600 mu l ammonia, and uniformly stirring, placing the reactant in a microwave oven to react for 3-7 minutes to obtain black suspension, cooling to room temperature, filtering the black suspension liquid in filtering process, and using de-ionized water to repeat washing. USE - The three-dimensional graphene nano composite material is useful in chiral recognition of tryptophan (claimed). DETAILED DESCRIPTION - Preparing three-dimensional graphene nano-composite material comprises (i) performing the ultrasonic processing 30-50 mg graphene oxide in 15-25 ml de-ionized water for 0.5-1 hours to obtain the graphene oxide dispersion, placing in a container, adding 70-90 mg L-cysteine dissolving in the graphene oxide dispersion liquid, and dripping 400-600 mu l ammonia, and uniformly stirring, placing the reactant in a microwave oven to react for 3-7 minutes to obtain black suspension, cooling to room temperature, filtering the black suspension liquid in the filtering process, using de-ionized water to repeat washing, and freezing and drying for 20-30 hours obtaining three-dimensional graphene (3D-G) powder, and (ii) placing the 10-30 mg obtained in the step (i) of 3D-G powder ultrasonic processing for 20-40 minutes in 10-30 ml deionized water, dispersing completely, adding 22-24 mg 1-ethyl (3-dimethyl amidopropyl)-carbodiimide hydrochloride and 13-15 mg N-hydroxy succinimide, stirring mechanically at room temperature for 2-4 hours, adding the reaction solution in 400-800 mg 2-Hydroxypropyl- beta -cyclodextrin (HP- beta -CD), making the mixed liquid under mechanical stirring for 2-4 hours at 50-70 degrees C to obtain black suspension, cooling to room temperature, filtering, and washing by deionized water repeatedly, and finally freezing and drying for 20-30 hours to obtain the black 3D-G/HP powdered- beta -CD. An INDEPENDENT CLAIM is also included for use method of three-dimensional graphene nano-composite material for chiral recognition of tryptophan comprising (i) taking 3D-G/HP- beta -CD powder in mixed solution of ethanol and water, and ultrasonically processing to prepare the dispersion liquid of 1 mg/ml, and taking 5 mu l 3 D-G/HP- beta -CD dispersion coated on the polishing surface of the glassy carbon electrode, drying to obtain 3D-G/HP- beta -CD modified electrode, (ii) immersing 3D-G/HP- beta - CD modified electrode with 0.1 M potassium chloride and 5 mM potassium ferricyanide, adding 5mM in the mixed solution of potassium ferricyanide (pH 7), respectively executing the electrochemical impedance and cyclic voltammetry characteristic, 3 D-G/HP- beta -CD modified electrode exhibits a low electrochemical impedance value and high peak current, (iii) immersing the 3D-G/HP- beta -CD modified electrode in the phosphate buffered saline buffer solution of 1mM-tryptophan at pH 7, performing differential pulse volt-ampere test, measuring the differential pulse volt-ampere of current response signal so as to judge the L-type and D-type of tryptophan, where the experimental results show that the current response signals of 3D-G/HP- beta -CD modified electrode in L-tryptophan and D-tryptophan solutions are obviously different, and the current response value of L-tryptophan is higher than D-tryptophan response value of the current, and using this difference to distinguish chiral inclusion tryptophan is L type or D type.