▎ 摘　　要

NOVELTY - Enzyme activity detecting solution comprises enzymes comprising endonuclease, helicase and DNA methyltransferase, double-stranded nucleic acid containing fluorescent dyes at single-strand ends, and graphene oxide. USE - The enzyme activity detecting solution is useful in preparing enzyme inhibitor screening kit (claimed). ADVANTAGE - The enzyme activity detecting solution and the method quantitatively monitor enzyme activity in real time without additional reagents, are cost-efficient, and make use of graphene oxide having longer storage stability. The kit screens enzyme inhibitors in easy manner, and exhibits high-throughput screening. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for: (1) detecting activity of enzymes including endonuclease, helicase and DNA methyltransferase using graphene oxide, comprising measuring fluorescence emission from enzymes, double-stranded nucleic acid containing fluorescent dyes at single-strand ends, and graphene oxide at regular intervals of time; and (2) enzyme inhibitor screening, comprising measuring fluorescence emission from mixture comprising enzyme inhibitor candidate material and enzyme activity detecting solution, where the mixture has different enzyme inhibitor candidate materials in different concentrations.