▎ 摘 要
NOVELTY - Preparing reduced graphene/conductive polymer/metal sulfide ternary composite material comprises e.g. (i) adding 0.5-2 g graphite and 0.5-2 g sodium nitrate into 50-100 ml concentrated sulfuric acid to obtain oxidized graphite, (ii) dispersing 0.02-0.1 g graphite oxide and 0.1-1 g metal salt in 50-100 ml isopropanol, and (iii) dispersing 20-30 mg graphite oxide/metal oxide binary nanocomposite in 20-30 ml distilled water, adding 100-150 mu l conductive polymer monomer, stirring, then adding 0.05-0.1 g thiourea, then performing hydrothermal reaction, centrifugal washing, and drying. USE - The reduced graphene/electro-conductive polymer/metal sulfide ternary composite material is useful as peroxidase catalyst (claimed). ADVANTAGE - The method is simple and product has stable morphology and good catalytic activity on catalytic oxidation of peroxidase. DETAILED DESCRIPTION - Preparing reduced graphene/conductive polymer/metal sulfide ternary composite material comprises (i) adding 0.5-2 g graphite and 0.5-2 g sodium nitrate into 50-100 ml concentrated sulfuric acid at a concentration of 95-98 wt.%, then adding 10-20 g potassium permanganate and stirring at 35-40 degrees C for 1-2 hours, adding 100-300 ml distilled water to the solution, stirring under 90-95 degrees C for 30-60 minutes, adding 200-800 ml distilled water and 5-20 ml water into the solution, and adding 30% peroxide, centrifugating, washing with water until pH is 6-7, and drying centrifuged product to obtain oxidized graphite, (ii) dispersing 0.02-0.1 g graphite oxide and 0.1-1 g metal salt in 50-100 ml isopropanol for 2-3 hours, taking 25-35 ml dispersion liquid at 80-100 degrees C for 30-60 minutes, adding 5-7.5 ml distilled water and continue heating and refluxing for 30-60 minutes, washing the mixture with water after centrifugation, and drying the product to obtain graphite oxide/metal oxide(MxOy) binary nanocomposite, and (iii) dispersing 20-30 mg graphite oxide/metal oxide(MxOy) binary nanocomposite in 20-30 ml distilled water, adding 100-150 mu l conductive polymer monomer, stirring at 0-30 degrees C for 2.5-3 hours, then adding 0.05-0.1 g thiourea, stirring at 10-30 degrees C for 0.5-1 hour, then performing hydrothermal reaction at 120-150 degrees C for 4-8 hours, centrifugal washing, and drying centrifugation product to obtain final product. M = copper, iron, cadmium, molybdenum or tungsten; x = 1 or 2; and y = 1, 2, or 3. INDEPENDENT CLAIMS are also included for: (1) reduced graphene/conductive polymer/metal sulfide ternary composite material obtained by above method; and (2) use method of reduced graphene/electro-conductive polymer/metal sulfide ternary composite material as peroxidase catalyst comprising carrying out ultrasonic dispersion of ternary composite material into water to form aqueous dispersion having concentration of 1-3 mg/ml, adding 10-50 mu l 1-5 ml acetic acid buffer solution, 10-20 mM 3,3',5,5'-tetramethylbenzidine in dimethyl sulfoxide and 10-50 mu l 1-100 mM peroxide aqueous solution, then adding 10-50 mu l above aqueous dispersion and catalyzing catalytic properties of peroxidase by ultraviolet light-visible absorption spectroscopy.