▎ 摘　　要

NOVELTY - Producing 2D nano-structured hybrid material of polypyrrole-polypyrrole carboxyl copolymer (PPy-PPa) and an electrochemically reduced graphene oxide (erGO) applied for manufacturing a biological sensor for early diagnosis, involves fixing a monoclonal antibody to PPy-PPa/erGO/screen-printed carbon electrode (SPCE) via a valence bond between the amino group of an antibody and the carboxyl group of the polymer; taking functional hydroxyl groups (-OH) on the surface and methyl group (-CH3) attached to the edges of erGO monolayers to function as oxidation targets in PPy-PPa polymerization reaction resulting in the PPy-PPa chain bound to the erGO, thus forming a two-dimensional nano-plate structure; tightly attaching the PPy-PPa on the ergo via a stacking interaction pi - pi ; and expanding and contracting due to the stimulation or non-stimulation provided by a network of erGO plates. USE - The method is useful for producing 2D nano-structured hybrid material of PPy-PPa and erGO applied for manufacturing a biological sensor for early diagnosis (claimed). ADVANTAGE - The method can provide stable development of the PPy-PPa polymer film which increases the repeatability of the sensor. In addition, the erGO provides a conductive network under the PPy-PPa polymer layer, facilitating the electronic transfer between the PPy-PPa and the erGO, which increases the sensitivity of the sensor.