▎ 摘　　要

NOVELTY - High-efficiency detecting ascorbic acid mimetic enzyme comprises iron-nickel sulfide (Fe0.8Ni0.2S2) and graphene, where the iron-nickel sulfide (Fe0.8Ni0.2S2) is uniformly dispersed on the graphene sheet layer, is new. USE - High-efficiency detecting ascorbic acid mimetic enzyme used as preservative in food, cosmetic and medicine for cold, mental disease, atherosclerosis, scurvy, cancer and other diseases. The Fe0.8Ni0.2S2/GO mimic enzyme can be used to detect ascorbic acid at room temperature with short detection time and high selectivity. ADVANTAGE - The method provides high-efficiency detecting anti-necrosis enzyme with improved dispersibility and conductive ability of metal sulfide, so as to improve the activity of the analogue enzyme, high efficiency at room temperature, and rapid detecting ascorbic acid. The graphene in the simulation enzyme can effectively prevent agglomeration of iron nickel sulfide (Fe0.8Ni0.2S2) nanoparticles, change the content of iron and nickel of different oxidation number, increase the electron transfer, and improve the enzymatic activity. The iron nickel sulfide (Fe0.8Ni0.2S2)/graphene oxide (GO) simulation enzyme is simple, the needed raw material source is wide, the price is low, which is excellent for saving cost. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for a preparation method of high-efficiency detecting ascorbic acid mimetic enzyme, involving (a) dissolving iron(III) nitrate nonahydrate, nickel nitrate hexahydrate and sodium sulfide nonahydrate in water, and adding a graphene aqueous solution containing sodium dodecylbenzenesulfonate to obtain a mixed solution, (b) keeping the mixed solution at constant temperature for 10-20 hours under the condition of 100-150degrees Celsius to react, and (c) cooling the reaction mixture obtained in step (b) to room temperature, centrifuging to obtain a solid product, alternately washing with deionized water and ethanol, and finally performing vacuum drying at 50-80degrees Celsius for 20-30 hours to obtain the enzyme.