▎ 摘　　要

NOVELTY - Constructing an electrochemical aptamer sensor based on diblock deoxyribonucleic acid involves preparing poly-C-containing diblock deoxyribonucleic acid-graphene oxide complex by adding the poly-C-containing diblock deoxyribonucleic acid sequence and graphene oxide to the buffer solution with pH to 7.5, reacting at room temperature for 10-12 hours, centrifuge, and washing to obtain the poly-C-containing diblock DNA-graphite oxide ene complex, ultrasonically dispersing the acidified multi-walled carbon nanotubes in the chitosan solution, and applying it to the surface of the glassy carbon electrode by a drop coating method, and drying naturally, applying prepared poly-C-containing diblock DNA-graphene oxide composite to the surface of prepared modified glassy carbon electrode, sealing and drying at 0-4 degrees C. The bovine serum albumin is added with 1-2 wt.% to the surface of prepared modified glassy carbon electrode. USE - Method for constructing electrochemical aptamer sensor based on diblock deoxyribonucleic acid used for detecting kanamycin (claimed). ADVANTAGE - The method enables to construct an electrochemical aptamer sensor based on diblock deoxyribonucleic acid is simple and does not require any markers to modify it, cost-effective, and realizes sensitive, rapid and efficient detection of kanamycin. DETAILED DESCRIPTION - Constructing an electrochemical aptamer sensor based on diblock deoxyribonucleic acid involves preparing poly-C-containing diblock deoxyribonucleic acid-graphene oxide complex by adding the poly-C-containing diblock deoxyribonucleic acid sequence and graphene oxide to the buffer solution with pH to 7.5, reacting at room temperature for 10-12 hours, centrifuge, and washing to obtain the poly-C-containing diblock DNA-graphite oxide ene complex, ultrasonically dispersing the acidified multi-walled carbon nanotubes in the chitosan solution, and applying it to the surface of the glassy carbon electrode by a drop coating method, and drying naturally, applying prepared poly-C-containing diblock DNA-graphene oxide composite to the surface of prepared modified glassy carbon electrode, sealing and drying at 0-4 degrees C. The bovine serum albumin is added with 1-2 wt.% to the surface of prepared modified glassy carbon electrode, and incubated at room temperature for 1-2 hours to obtain double embedded Segmented DNA electrochemical aptamer sensor. An INDEPENDENT CLAIM is included for a method for using diblock DNA-based electrochemical aptamer sensor to detect kanamycin, which involves adding phosphate buffer containing 5.0 millimole ferricyanide anion and 0.1 mole potassium chloride at a concentration of 0.2 mole and pH of 7.4 in the liquid under the condition that the voltage of the electrochemical workstation is (-)0.1-0.4 volt, and using differential pulse voltammetry for detection, where current has a good linear relationship with the logarithm of kanamycin concentration in the range of kanamycin concentration from 0.05 picomolar-100 nanomolar.