• 专利标题:   Detecting target nucleic acids by mixing single-stranded template with graphene or graphene oxide, adding e.g. cleavage enzyme, performing exponential isothermal amplification reaction, and confirming presence of nucleic acid.
  • 专利号:   KR2018001893-A, KR1820440-B1
  • 发明人:   JANG H, PARK H G, PARK J H
  • 专利权人:   KOREA ADVANCED INST SCI TECHNOLOGY, KOREA ADVANCED SCI TECHNOLOGY INST
  • 国际专利分类:   C12Q001/68, G01N027/62
  • 专利详细信息:   KR2018001893-A 05 Jan 2018 C12Q-001/68 201815 Pages: 10
  • 申请详细信息:   KR2018001893-A KR080961 28 Jun 2016
  • 优先权号:   KR080961

▎ 摘  要

NOVELTY - Method for detecting target nucleic acids involves (a) mixing a single-stranded template comprising a sequence complementary to the target nucleic acid with graphene or graphene oxide, and adding deoxynucleotide triphosphates (dNTPs), DNA polymerase, cleavage enzyme and to-be-analyzed sample, and performing an exponential isothermal amplification reaction (EXPAR), and (b) confirming the presence of the target nucleic acid by mass analysis of the amplification reaction product. USE - The method is useful for detecting target nucleic acids. ADVANTAGE - The method detects target nucleic acids in a simple, rapid and accurate manner without labeling the target nucleic acid, and enables to prevent and diagnosis diseases at an early stage.