▎ 摘　　要

NOVELTY - Preparation of double-output mode biosensor involves preparing self-enhanced electrochemiluminescent material dispersion, grinding glassy carbon electrode with aluminum oxide powder, ultrasonically processing in ethanol and water, drying to obtain treated glassy carbon electrode, adding self-enhanced electrochemiluminescent material dispersion to modify surface of treated glassy carbon electrode, fixing with Nafion (RTM: sulfonated tetrafluoroethylene based fluoropolymer-copolymer) film to obtain modified glassy carbon electrode, using gold nanoparticles to modify surface of modified glassy carbon electrode to obtain gold nanoparticle-graphene quantum dots doped silicon dioxide/glassy carbon electrode composite material, using complementary chain of aptamer modified by sulfhydryl group as complementary DNA, fixing complementary DNA on electrode surface, heat preserving and using ferrocene-labeled aflatoxin B1 (AFB1) aptamer to doped on electrode surface of material. USE - Preparation of double-output mode biosensor for detection of aflatoxin B1 (all claimed). ADVANTAGE - The prepared double-output mode electrochemiluminescent biosensor has high stability, high selectivity, high sensitivity and low interference in detection of aflatoxin B1, and can be used for sensitive and rapid analysis of actual samples. DETAILED DESCRIPTION - Preparation of double-output mode biosensor involves mixing terpyridine ruthenium aqueous solution with graphene quantum dot aqueous solution, stirring in dark condition, adding cyclohexane, Triton (RTM: t-octyl phenoxy polyethoxy ethanol) and hexanol, stirring, adding tetraethyl silicate and ammonia, sealing and stirring, adding to acetone, centrifuging, washing solids with ultrapure water and ethanol to obtain ruthenium and nitrogen co-doped graphene quantum dots doped silicon dioxide, dispersing in ultrapure water to obtain self-enhanced electrochemiluminescent material dispersion, grinding glassy carbon electrode with aluminum oxide powders of different particle sizes, ultrasonically processing in ethanol and water for 30 seconds, air drying to obtain treated glassy carbon electrode, adding self-enhanced electrochemiluminescent material dispersion to modify the surface of treated glassy carbon electrode and fixing with Nafion (RTM: sulfonated tetrafluoroethylene based fluoropolymer-copolymer) film to obtain modified glassy carbon electrode, using gold nanoparticles to modify the surface of modified glassy carbon electrode, naturally drying at room temperature to obtain gold nanoparticle-graphene quantum dots doped silicon dioxide/glassy carbon electrode composite material, taking complementary chain of the aptamer modified by sulfhydryl group and using as complementary DNA, fixing complementary DNA on electrode surface of material, heat preserving, and using ferrocene-labeled aflatoxin B1 (AFB1) aptamer to doped on electrode surface of heat preserved material to form double-output mode sensor based on ferrocene-labeled aptamer amplifying electrochemiluminescence signal. An INDEPENDENT CLAIM is included for application method of the double-output mode sensor for detection of aflatoxin B1, which involves using the double-output mode sensor, AFB1 solution and tris(hydroxymethyl)aminomethane hydrochloride solution.