▎ 摘　　要

NOVELTY - Preparation of fluorescent polystyrene microspheres involves adding concentrated nitric acid and concentrated sulfuric acid to round bottom flask containing pyrene, reacting, cooling to room temperature, diluting with ultrapure water, filtering under reduced pressure to obtain a golden yellow product, drying in an electric heating constant temperature blast drying oven at 60℃ to obtain trinitropyrene (TNP), ultrasonically dispersing TNP in toluene to obtain uniform suspension, adding to PTFE-lined stainless steel high-pressure reactor, reacting at 100-180℃, naturally cooling, filtering using organic filter membrane and rotary evaporating the filtrate to obtain reddish-brown oxidized graphene quantum dots (o-CQDs), and dissolving o-CQDs in styrene to obtain a solution (A), mixing solution (A) and styrene to obtain solution (B), mixing solution (B) with styrene to obtain solution (C), and reacting with methacrylic acid in presence of ammonium persulfate and sodium bicarbonate. USE - Preparation of fluorescent polystyrene microspheres for detecting living organisms (claimed) and in vivo tracking. ADVANTAGE - The prepared fluorescent microspheres have high biocompatibility, low toxicity, and excellent hydrophilicity, water dispersibility and anti-interference ability to co-existing metal ions such as ferric ions, potassium ions, divalent barium, divalent zinc, divalent magnesium, divalent calcium and sodium ions in the environment, and strong fluorescence stability. The microspheres do not increase the toxicity of polystyrene and the ability of polystyrene to enter the brine shrimp is proved by fluorescent tracer. DETAILED DESCRIPTION - Preparation of fluorescent polystyrene microspheres involves adding 60 mL concentrated nitric acid and 20 mL concentrated sulfuric acid to a round bottom flask containing 0.5-2 g pyrene, placing in an oil bath at 40-80℃, reacting, cooling to room temperature, diluting with 50 mL ultrapure water, filtering under reduced pressure to obtain a golden yellow product, drying in an electric heating constant temperature blast drying oven at 60℃ to obtain trinitropyrene (TNP), ultrasonically dispersing 0.1 g TNP in toluene to obtain uniform suspension, adding to PTFE-lined stainless steel high-pressure reactor, reacting at 100-180℃, naturally cooling, filtering using organic filter membrane and rotary evaporating the filtrate to obtain reddish-brown oxidized graphene quantum dots (o-CQDs), and dissolving 0.06 g o-CQDs in 15 mL styrene to obtain a solution (A), mixing 2 mL solution (A) and 3 mL styrene to obtain solution (B), mixing 3 mL solution (B) with 2 mL styrene to obtain solution (C), and reacting at 40-80℃ with 0.50 mL methacrylic acid (MMA) in presence of 0.05-0.50 g ammonium persulfate (APS) and 0.12 g sodium bicarbonate. The total amount of o-CQDs and styrene are 5.00 mL.