▎ 摘　　要

NOVELTY - Electrochemiluminescence detection kit based on GO-CDTPA-AuNPs and photo-induced electron/energy transfer-reversible additon-fragmentation chain transfer (PET-RAFT) comprises gold electrode, hairpin DNA (hDNA), 6-Mercapto-1-hexanol (MCH), 3-mercaptopropionic acid-N-hydroxysuccinimide (MPA-NHS) ester, graphene oxide-4-cyano-4-(dodecylsulfanylthiocarbonyl) sulfanyl pentanoic acid-gold nano particles (GO-CDTPA-AuNPs), tris(2-dimethylaminoethyl)amine (Me6TREN), eosin Y (EY), N-acryloxysuccinimide (NAS), and Luminol. USE - The electrochemiluminescence detection kit is useful in preparing detection reagent for detecting lung cancer, preferably non-small cell lung cancer (all claimed). ADVANTAGE - The detection kit: avoids use of biological enzymes in current common signal amplification strategy, and multiplied signal, which has improved detection sensitivity, stability and reproducibility. The method: avoids instability of luminol combined with GO through conjugation; has improved surface utilization of GO, improved ECL efficacy of luminol; utilizes combination of GO and AuNPs, which avoids disadvantages of unstable combination and easy aggregation of traditional nanomaterials; adopts light-initiated RAFT polymerization, which avoids thermal initiation in traditional RAFT reactions, which has mild reaction conditions, faster reaction times, and living/controllable polymerization at room temperature; and is biological toxicity free, and environmentally friendly. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: use method of electrochemiluminescence detection kit, comprising (i) polishing bare gold electrode to obtain polished mirror surface, (ii) (a) dropping hDNA solution on electrode surface and reacting, (b) immersing electrode into MCH solution and reacting, (c) dropping solution to be tested directly on electrode surface, and reacting, (d) soaking electrode in MPA-NHS ester solution and reacting, (e) dropping GO-CDTPA-AuNPs dispersion on electrode surface, and reacting, (f) placing electrode in mixed solution contains Me6TREN solution, EY solution, NAS solution and phosphate buffered saline buffer solution, and reacting, (g) placing electrode in luminol solution and reacting, and (h) placing the electrode in hydrogen peroxide to measure luminescence intensity of luminol. use of detection kit in preparing detection reagent for detecting lung cancer.