▎ 摘　　要

NOVELTY - Preparing enzyme-free glucose sensor comprises e.g. preparing reductive graphene oxide aqueous solution, treating ultrasonically for 30 minutes, adding chitosan solution, then mixing with nano iron(III) oxide solution, treating ultrasonically for 5-15 minutes, grinding, cleaning with ultrapure water, air drying with nitrogen gas, adding reductive graphene oxide-iron(III) oxide nanocomposites, drying, infiltrating, drying with nitrogen gas, drying, adding potassium hexachloroplatinate (iv) hexahydrate, potassium chloride and phosphate buffered saline solution, mixing and drying. USE - The method is useful for preparing enzyme-free glucose sensor. ADVANTAGE - The enzyme-free glucose sensor has high sensitivity, excellent repeatability and stability and anti-interference ability. DETAILED DESCRIPTION - Preparing enzyme-free glucose sensor comprises (i) preparing reductive graphene oxide aqueous solution (RGO), treating ultrasonically for 30 minutes, and adding chitosan solution (CS) in a volume ratio of 2-3:1, then treating ultrasonically for 1-2 hours, then removing part of the mixed solution, then mixing with nano iron oxide Fe2O3 solution in a volume ratio of 10-12:1, treating ultrasonically for 5-15 minutes to obtain reductive graphene oxide-iron(III) oxide nanocomposites, (ii) taking modified electrode tip having a diameter of 3-6 mm and alumina powder having a diameter of 0.3-0.05 mu m, grinding on the suede until the tip surface presents a mirror-smooth, cleaning with ultrapure water for 5-10 minutes, air drying with nitrogen gas, adding 1.5-3 mu l reductive graphene oxide-iron(III) oxide nanocomposites, dropping to be polished electrode surface polished smooth, drying at room temperature for greater than or equal to 3 hours, then infiltrating the modified dried modified electrode with distilled water for 2 minutes, drying with nitrogen gas, drying, repeatedly infiltrating and drying for 2-3 times, (iii) taking the first modified spare electrode, adding potassium hexachloroplatinate (iv) hexahydrate, potassium chloride and phosphate buffered saline solution, mixing at the potential window of -0.4 to +0.4 V, the scanning speed is 50 mV/s, scanning for 5-20 laps, then washing the modified electrode with a large amount of deionized water, drying at room temperature, and using the modified electrode as a sensor for the electrochemical analysis of glucose.