▎ 摘　　要

NOVELTY - Preparing a bioelectrode involves modifying graphite electrode with electrochemically graphene oxide, which is followed by electrochemical polymerization of poly(3-hydroxybenzoic acid) polymer for incorporation of oligonucleotide probes, carrying out immobilization by adsorption of probe oligonucleotide having 20 nucleobases (SEQ ID NO: Not defined), given in the specification, detecting complementary target having 20 nucleobases (SEQ ID NO: Not defined), given in the specification in direct real time, monitoring oxidation peak of guanine nitrogen base, using the differential pulse voltammetry technique, or indirectly real time, and using Hoechst 33258 indicator per half-hour differential pulse voltammetry technique. USE - Method for preparing a bioelectrode for detecting Alicyclobacillus acidoterrestris bacterium in orange juice. ADVANTAGE - The method enables to prepare a bioelectrode, which has low cost and analyzes real sample. DETAILED DESCRIPTION - Preparing a bioelectrode involves modifying graphite electrode with electrochemically graphene oxide, which is followed by electrochemical polymerization of poly(3-hydroxybenzoic acid) polymer for incorporation of oligonucleotide probes, carrying out immobilization by adsorption of probe oligonucleotide having 20 nucleobases (SEQ ID NO: Not defined), which are 5'-ctgtgttgatgttgttggcg-3', detecting complementary target having 20 nucleobases (SEQ ID NO: Not defined), which are 5'-cgccaacaacatcaacacag-3' in direct real time, monitoring oxidation peak of guanine nitrogen base, using the differential pulse voltammetry technique, or indirectly real time, and using Hoechst 33258 indicator per half-hour differential pulse voltammetry technique. The method involves detecting genomic DNA of Alicyclobacillus acidoterrestris present in a cell lysate indirectly and in real time, and using indicator Hoechst 33258 by technique of differential pulse voltammetry in phosphate buffer solution or contaminated orange juice.