▎ 摘　　要

NOVELTY - The method comprises configuring (805) a reservoir separated into two parts by a membrane, configuring (810) a nanopore formed through the membrane, where the nanopore connects the two parts of the reservoir, and filling (815) the nanopore and the two parts of the reservoir with ionic buffer. The membrane comprises a graphene layer and insulating layers. The graphene layer is wired to first and second metal pads to form a graphene transistor in which transistor current flowing through the graphene transistor is modulated by charges or dipoles passing through the nanopore. USE - The method is useful for electrically differentiating bases or identifying biomolecules (claimed) in a nanopore using a graphene nanopore transistor, where the bases include DNA nucleobases. ADVANTAGE - The method is capable of accurately differentiating bases or identifying biomolecules in the nanopore with high spatial resolution using the high sensitivity graphene nanopore transistor. DETAILED DESCRIPTION - The method comprises configuring (805) a reservoir separated into two parts by a membrane, configuring (810) a nanopore formed through the membrane, where the nanopore connects the two parts of the reservoir, and filling (815) the nanopore and the two parts of the reservoir with ionic buffer. The membrane comprises a graphene layer and insulating layers. The graphene layer is: wired to first and second metal pads to form a graphene transistor in which transistor current flowing through the graphene transistor is modulated by charges or dipoles passing through the nanopore; and formed with a left graphene part and a right graphene part connected by a narrowed neck. The nanopore is formed through the narrowed neck. The charges or dipoles correspond to a nucleobase of a nucleic acid molecule and to a biomolecule. The transistor current modulates based on the charges or dipoles of the nucleobase, when the nucleobase is in the nanopore, to form a transistor current pulse, and the transistor current modulates based on the charges or dipoles of the biomolecule, when the biomolecule is in the nanopore, to form a transistor current pulse. The method further comprises determining the nucleobase based on a magnitude, a time duration and a shape of the transistor current pulse when the nucleobase is in the nanopore, and determining the biomolecule based on the magnitude, the time duration and the shape of the transistor current pulse when the biomolecule is in the nanopore. An ionic current is changed based on a size of the biomolecule, when a voltage is applied across the nanopore, to form an ionic current pulse. The biomolecule is determined based on a magnitude and a time duration of the ionic current pulse when the biomolecule is in the nanopore. The nanopore is coated with an organic layer configured to interact with the nucleobase differently than other nucleobases and interact with the biomolecule differently than other biomolecules. DESCRIPTION OF DRAWING(S) - The diagram shows a flow chart of a method for differentiating and identifying bases and biomolecules in a nanopore. Differentiating and identifying bases and biomolecules in a nanopore (800) Configure a reservoir separated into two parts by a membrane (805) Configure a nanopore formed through the membrane (810) Fill the nanopore and the two parts of the reservoir with ionic buffer (815) Wiring the graphene layer to metal pads to form a graphene transistor. (820)