▎ 摘　　要

NOVELTY - A copper(II) oxide-polylysine/graphene/substrate electrode preparing method involves removing a surface oxide of a substrate, drying, adding nickel(II) nitrate ethanol solution on the surface of the substrate and baking. The substrate is subjected to direct current plasma jet chemical vapor deposition and performed an arc discharge, sprayed methane, and terminated the direct current vapor jet chemical vapor deposition to obtain graphene/ a substrate electrode, inserted the graphene/substrate electrode and the counter electrode into an electrolyte, and selected a pulse wave to electrodeposit the graphene/substrate electrode, obtained a copper-polylysine/graphene/substrate electrode, inserted copper-polylysine/graphene/substrate electrode, counter electrode and reference electrode into an aqueous sodium hydroxide solution, oxidized copper by cyclic voltammetry, taken out the copper-polylysine/graphene/substrate electrode and rinsed with water to obtain the finished product. USE - Method for preparing copper(II) oxide-polylysine/graphene/substrate electrode for detection of melatonin and pyridoxine (claimed). ADVANTAGE - The method enables preparing copper(II) oxide-polylysine/graphene/substrate electrode with better reproducibility, repeatability and stability of the sensor, low detection limit, high test sensitivity and accuracy, low cost, better for civil, and in simple and convenient manner. DETAILED DESCRIPTION - A copper(II) oxide-polylysine/graphene/substrate electrode preparing method involves removing a surface oxide of a substrate, drying after washing, adding dropwise 100-300 mu L nickel(II) nitrate ethanol solution on the surface of the substrate, and baking under an infrared lamp for 2-3 minutes for drying. The substrate is subjected to direct current plasma jet chemical vapor deposition by cleaned reaction chamber, cleaned the substrate in the reaction chamber, evacuated the reaction chamber, introduced an inert gas and hydrogen, and performed an arc discharge, after 4-6 minutes of arc discharge, sprayed methane into the reaction chamber for 5-30 minutes under the condition of maintaining arc discharge, and terminated the direct current vapor jet chemical vapor deposition to obtain graphene/ a substrate electrode, inserted the graphene/substrate electrode and the counter electrode into the electrolyte, and selected a pulse wave to electrodeposit the graphene/substrate electrode while maintaining the electrolyte stirring state, after electrodeposition obtained a copper-polylysine/graphene/substrate electrode, inserted three-electrode system consisting of copper-polylysine/graphene/substrate electrode, counter electrode and reference electrode into an aqueous sodium hydroxide solution, oxidized copper by cyclic voltammetry by setting a potential range to 0-1 V, a voltage scanning speed to 0.01-0.1 V/s and scanning is 50-100 cycles, taken out the copper-polylysine/graphene/substrate electrode and rinsed with water to obtain the finished product, where the substrate is a ruthenium, titanium or molybdenum sheet, and the flow rate of methane is 200-300 mL/min, the arc voltage is 70-80 V, and the arc current is 110-120 A. INDEPENDENT CLAIMS are also included for the following: (1) a copper(II) oxide-polylysine/graphene/substrate electrode prepared by the method (2) use of copper(II) oxide-polylysine/graphene/substrate electrode for detection of melatonin and pyridoxine.