▎ 摘　　要

NOVELTY - Electrode patch comprises anode patch and cathode patch, where the anode patch comprises a first electrode substrate (11), first working electrode (12) set on the first electrode substrate, the first reference electrode (13) and the first pair of electrodes (14), the cathode patch comprises a second electrode substrate (21), second working electrode set on the second electrode substrate, the second reference electrode and the second pair of electrodes, the first working electrode, the first reference electrode, the first pair of electrodes, the second working electrode, the second reference electrode and the second pair of electrodes respectively connected with lead, the lead is covered with an insulating layer, the tail end of the lead is provided with a pin. USE - Electrode patch used for non-invasive blood glucose detection in biological liquid such as sweat, saliva, urine and tissue fluid. ADVANTAGE - The non-invasive blood sugar detecting electrode patch is an electrochemical biosensor, extracting human body tissue fluid by reverse ion electroosmosis method, so as to realize quantitative noninvasive detection of glucose by using timing current method and electrochemical measuring technology. The hydrogel layer is agarose solution with excellent biocompatibility. The first electrode substrate and the second electrode substrate are a PET film, the anode patch and cathode patch manufactured by screen printing. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: 1. Manufacturing method of non-invasive blood sugar detecting electrode patch, involving: a. using screen printing technology, using ink printing lead and pin on the first electrode substrate, the first pair of electrodes and the first working electrode, the first reference electrode, insulating layer, performing curing treatment at a certain temperature to obtain the anode patch; b. using screen printing technology, using ink printing lead and pin on the second electrode substrate, the second pair of electrodes and the second working electrode, the second reference electrode, insulating layer, and curing treatment at a certain temperature to obtain the semi-finished product cathode patch; c. brushing a layer of silver paste ink on the second working electrode of the semi-finished product cathode patch, putting in the oven to solidify to semi-solidified state, then uniformly dropping 10 microLiter of graphene solution containing Prussian blue on the silver paste ink, putting into 40degrees Celsius water bath to heat for 12 hours d. cleaning the semi-finished product cathode patch after water bath heating with pure water, drying by air after drying; e. uniformly dropping 5 microLiter chitosan-glutaraldehyde and glucose oxidase mixed solution to the second working electrode and then placing in the refrigerator for drying at -4degrees Celsius; f. coating agarose hydrogel on the second working electrode, the second reference electrode and the second pair of electrodes to obtain product; and 2. Reverse iontophoresis in vitro experiment device, comprises a diffusion cell, isolated pigskin, a lead, a magnetic stirrer, a stirrer, an electrochemical working station and the non-invasive blood glucose detection electrode patch, the diffusion cell comprises a sample cell, a sample inlet and a placing platform, the sample pool is set on the magnetic stirrer, the sample inlet is connected with the sample pool, the sample pool is filled with sample solution. DESCRIPTION OF DRAWING(S) - The drawing shows a schematic view of a patch. Insulating layer (4) Pin (5) Hydrogel layer (6) First electrode substrate (11) First working electrode (12) First pair of electrodes (14) Second electrode substrate (21)