▎ 摘　　要

NOVELTY - Preparing fluorescent sensor comprises (i) mixing citric acid and glutathione, heating and liquefying, dialyzing, evaporating and drying after dialyzing to obtain a solid, and dissolving the solid to obtain glutathione functionalized graphene quantum dot mother liquid, and (ii) diluting glutathione functionalized graphene quantum dots mother liquid, sequentially adding ferric chloride, Tris-hydrochloric acid buffer solution and phytic acid, uniformly mixing, then adding acetylcholine, acetylcholinesterase and choline oxidase, uniformly mixing, and incubating at 37°C for 60 minutes to obtain graphene quantum dots doped GSH/iron(III) ion/polyamide/(ACh+AChE+ChOx) fluorescent sensor. USE - The fluorescent sensor is useful for detecting Angelica organophosphorus pesticide residues (claimed). ADVANTAGE - The method: is based on the principle of polyethylene terephthalate; utilizes QDs as basic material to construct a new type of optical fluorescence sensor that specifically recognizes organophosphorus pesticide residues in Angelica; can focus on structural design and synthesis of fluorescent probe molecules, fluorescence enhancement effects, selectivity and sensitivity of fluorescence detection; identifies fluorescent probes and mechanism of fluorescent response; is simple, fast and accurate; and realizes in-situ real-time dynamic fluorescence detection and imaging analysis of organic pesticide residues in Chinese herbal medicines. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for a method for detecting Angelica organic phosphorus pesticide residue by using the fluorescent sensor, comprising (a) detecting organic phosphorus content in standard solution by taking 7 copies of 9 ml graphene quantum dots doped GSH/iron(III) ion/polyamide/(ACh+AChE+ChOx) fluorescent sensor, adding standard solution sample of muscarinic acid with concentration gradients of 0.1, 0.2, 0.5, 1, 2, 5 and 10 μmol.L-1 respectively, uniformly mixing, incubating at room temperature for 0-100 minutes, measuring the fluorescence intensity of standard solution sample at emission wavelength 340-550 nm under the excitation of 342 nm, placing with fluorescence spectrophotometer, and establishing a linear relationship diagram according to the relationship between the concentration of the standard solution sample and the relative fluorescence intensity F/F0, (b) preparing samples to be tested by precisely weighing 5 g Angelica powder, placing in a 50 ml conical flask with a plug, using 25 ml methanol for ultrasonically extracting the sample twice, where each time extracting for 20 minutes, combining the extracts and filtering through filter paper, then concentrating the filtrate to dryness under reduced pressure, and re-dissolving the residue in 50 ml 100 μmol.L-1 muscarinic acid solution to obtain the sample to be tested, and (c) detecting organic phosphorus content in the sample to be tested by taking 4 copies of 9 ml graphene quantum dots doped GSH/iron(III) ion/polyamide/(ACh+AChE+ChOx) fluorescent sensor, adding the samples to be tested with concentration gradients of 0, 0.5, 1 and 2 μmol.L-1 respectively, uniformly mixing, incubating at 37°C for 80 minutes, measuring the fluorescence intensity at the emission wavelength 340-550 nm under excitation at 342 nm, placing the standard solution sample with a fluorescence spectrophotometer, and calculating the content of organic phosphorus in the sample solution according to the obtained standard linear relationship diagram.