▎ 摘　　要

NOVELTY - Self-priming valve compartmentalized chip comprises support layer and a reaction layer arranged on the support layer. A number of fluid microchannels and reaction microcavities are arranged on the reaction layer, and a sample inlet is also arranged in the middle of the reaction layer. USE - Self-priming valve compartmentalized chip. ADVANTAGE - The self-priming valve compartmentalized chip completes the detection within 10 minutes, has a small coefficient of variation, minimum detection concentration is 46.8 colony forming unit/mL, and recovery rate of standard addition reaches 98.08%, high sensitivity, good stability. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: (1) a method for preparing a self-priming valve compartmentalized chip, which involves using CorelDRAWX8 to design the channel and chamber pattern of the self-priming valve compartmentalized chip, and then print it on a transparent film as a mask, using spin coater to coat the negative photoresist on coat a total of two layers on the silicon wafer, placing the silicon wafer on a single-sided lithography machine under the mask, adjusting the position of the mask so that the pattern is in the middle of the wafer, and baking the silicon wafer after exposure, rinsing the processed silicon wafer with a developer until the mold pattern appears completely, and baking mold again to obtain a mold, using trimethylchlorosilane to process the mold, mixing polydimethylsiloxane component pre-curing agent A and crosslinking agent B to remove bubbles, pouring into the mold, and screwing the bolt and nut to one end flush, and placing flush end down on the valve position of the mold, curing on the heating plate, removing the polydimethylsiloxane from the surface of the mold, using puncher to perforate the sample hole, applying plasma treatment to the channel surface of the polydimethylsiloxane and the surface of the glass sheet at the same time, and the treating surfaces are bonded to each other and baked and cured to form a self suction valve compartmentalized chip; and (2) a method for detecting Listeria monocytogenes based on a self-priming valve segmented chip, which involves adding graphite powder to the mixture of H2SO4 and sodium nitrate, stirring in an ice bath, and vigorous stirring, slowly adding potassium permanganate to the above mixture, continue stirring at 30-40 degrees C, and heating to 140-150 degrees C and stirring, adding deionized water to terminate the reaction system, neutralizing H2SO4 with sodium hydroxide in an ice bath, adjusting the pH to 5.5-6, and obtaining a light yellow solution that is filtered through ion filtration membranes to remove large particles, and dialyzing in a dialysis bag for 2-3 days to obtain graphene oxide, pre-treating of the self-priming valve compartmentalized chip, using transparent tape to cover the top of the chip and placing in a vacuum pump for degassing, mixing graphene oxide, FAM-modified Listeria monocytogenes specific aptamer and HNB as a negative control to make the mixture distributed under negative pressure through the substrate injection hole in the sample hole, removing scotch tape, placing on the heating plate and heating up. After the substrate is completely dry, reattach the tape on the chip surface and placing in a vacuum pump for vacuum processing, injecting sample to be tested and paraffin oil into the general sampling hole, and using paraffin oil to detection chamber is separated, and placing chip in the small animal imager to take fluorescence images.