▎ 摘　　要

NOVELTY - An electrochemical sensor preparation method involves ultrasonically dispersing 25 mg graphene oxide in 50 ml water, forming uniform graphene oxide suspension with concentration of 0.5 mg/ml, adding diluted ammonia with molar fraction of 5% to adjust pH of the suspension to 8, adding 2 g urea and 100 mg boric acid to the suspension under vigorous stirring conditions, stirring obtained mixture at room temperature for 30 minutes, transferring to Teflon (RTM: PTFE) lined reactor, reacting at 120 degrees C for 12 hours, cooling, centrifuging obtained product at 12000 rotations per minute, repeatedly washing with water and ethanol, vacuum drying at 60 degrees C to obtain nitrogen-boron-doped reduced graphene oxide complex followed by obtaining iron phthalocyanine-nitrogen-boron-doped reduced graphene oxide modified electrode. USE - Method for preparing electrochemical sensor that is utilized for detecting glutathione in human serum. ADVANTAGE - The method enables preparing highly responsive electrochemical sensor with high sensitivity and selectivity, better stability and reproducibility and large detection range. DETAILED DESCRIPTION - An electrochemical sensor preparation method involves ultrasonically dispersing 25 mg graphene oxide in 50 ml water, forming uniform graphene oxide suspension with concentration of 0.5 mg/ml, adding diluted ammonia with molar fraction of 5% to adjust pH of the suspension to 8, adding 2 g urea and 100 mg boric acid to the suspension under vigorous stirring conditions, stirring obtained mixture at room temperature for 30 minutes, transferring to Teflon (RTM: PTFE) lined reactor, reacting at 120 degrees C for 12 hours, cooling, centrifuging obtained product at 12000 rotations per minute, repeatedly washing with water and ethanol, vacuum drying at 60 degrees C to obtain nitrogen-boron-doped reduced graphene oxide complex, dissolving iron phthalocyanine in secondary water, ultrasonically processing at 10 KHz for 10-30 minutes, obtaining iron phthalocyanine solution with concentration of 0.5-5 mM, adding 0.2-2 mg/ml nitrogen and nitrogen-boron-doped reduced graphene oxide complex to the solution, ultrasonically processing at 40 KHz for 10-30 minutes to form dispersion, applying dispersion (4-10 mu l) on glassy carbon electrode, drying under infrared light for 5-30 minutes, removing loosely adsorbed nanocomposites by rinsing with secondary water and obtaining iron phthalocyanine-nitrogen-boron-doped reduced graphene oxide modified electrode. An INDEPENDENT CLAIM is also included for an iron phthalocyanine-nitrogen-boron-doped reduced graphene oxide modified electrode for target detection.