▎ 摘　　要

NOVELTY - Determining lead by regulated graphene quantum dot catalyzed active surface plasmon resonance with absorption spectroscopy comprises (i) preparing the lead standard solution system in the scale test tube, sequentially adding 2-60 mu l of 1 mu mol/l lead standard solution, 5-20 mu l of 0.15 mu mol/l lead aptamer and 50-150 mu l of 0.5 mg/l graphene quantum dots, mixing and allowing to stand for 10 minutes; adding 20-60 mu l of 0.01 mol/l hydrochloric acid, 100-200 mu l of 0.5 mol/l glucose and 80-180 mu l of 84 mu mol/l chloroauric acid (HAuCl4), and mixing uniformly with double distilled water. USE - The method is useful for determining lead by regulated graphene quantum dot catalyzed active surface plasmon resonance with absorption spectroscopy (claimed). ADVANTAGE - The method does not require to construct aptamer-probe complex process, is convenient, fast, includes nanoparticles without aggregation, the system is more stable, provides graphene quantum dot nano-enzyme catalysis and has high sensitivity. DETAILED DESCRIPTION - Determining lead by regulated graphene quantum dot catalyzed active surface plasmon resonance with absorption spectroscopy comprises (i) preparing the lead standard solution system in the scale test tube, sequentially adding 2-60 mu l of 1 mu mol/l lead standard solution, 5-20 mu l of 0.15 mu mol/l lead aptamer and 50-150 mu l of 0.5 mg/l graphene quantum dots, mixing uniformly and allowing to stand for 10 minutes; adding 20-60 mu l of 0.01 mol/l hydrochloric acid, 100-200 mu l of 0.5 mol/l glucose and 80-180 mu l of 84 mu mol/l chloroauric acid (HAuCl4), mixing uniformly with double distilled water volume to 1.5 ml, placing in a water bath and reacting at 75 degrees C for 18 minutes, removing the test tube, adopting ice water for cooling and stop the reaction; (ii) adopting step (i) without adding lead standard solution to prepare blank control solution system; (iii) based on the step (i), (ii) adding prepared lead standard solution system and blank control solution system into the cuvette, scanning by spectrophotometer to obtain the absorption spectrum of the system, measuring the absorbance at 530 nm and taking as value of A , and determining the absorbance value of the blank control solution system as A0, and calculating Delta A=A-A0; (iv) taking Delta A as the working curve of the relationship between lead concentration; (v) preparing sample solution based on step (i), where the added lead standard solution is replaced with the sample solution, and the absorbance value of the sample solution is determined as A sample based on the step (iii), and calculating by Delta A sample=A sample-A0; and (vi) based on the work curve of step (iv), calculating the sample solution lead content.