▎ 摘　　要

NOVELTY - A Escherichia coli detecting electrochemical immune sensor preparing method involves preparing graphene and nano-gold nanoparticle composite (GR-AuNPs) followed by preparing graphite and nanogold nano-particle composite (GR-EDOT-AuNPs) by taking GR-AuNPs nanocomposite with pure water, stirring for 30 minutes to make the mix uniform, adding EDOT and continuously stir, circulation voltammetry by -0.2. to 0.6 electric polymerization process, that is, a preparation GREDOT-AuNPs nanocomposite. , USE - Method for preparing electrochemical immune sensor for detecting Escherichia coli in food (claimed). DETAILED DESCRIPTION - A Escherichia coli detecting electrochemical immune sensor preparing method involves preparing graphene and nano-gold nanoparticle composite (GR-AuNPs) by taking graphite oxide, hydrazine hydrate, graphene, chloroauric acid solution and polyvinylpyrrolidone, adding in distilled water, gradually adding 3.5 mu l hydrazine hydrate of a dispersion liquid, placing in water bath for 2 hours, graphene of polyvinylpyrrolidone, gradually adding sodium borohydride solution, stirring for 30 minutes, centrifugal cleaning for 5 times, drying, and storing in the refrigerator, followed by preparing graphite and nanogold nano-particle composite (GR-EDOT-AuNPs) by taking GR-AuNPs nanocomposite with pure water, stirring for 30 minutes to make the mix uniform, adding EDOT and continuously stir, circulation voltammetry by -0.2. to 0.6 electric polymerization process, that is, a preparation GREDOT-AuNPs nanocomposite. An INDEPENDENT CLAIM is also included for a method for utilization of detecting Escherichia coli in food by using electrochemical immune sensor.