▎ 摘　　要

NOVELTY - Biosensor for detection of an analyte comprises: (a) (i) a nucleic acid substrate coupled to a first support and a nucleic acid cleaving enzyme; or (ii) a nucleic acid cleaving enzyme comprising a nucleic acid substrate coupled to a first support; and (b) a nucleic acid binding molecule coupled to a microzone on a second support. The nucleic acid substrate comprises a detection moiety. The nucleic acid enzyme is capable of cleaving the nucleic acid substrate. The nucleic acid cleaving enzyme is activated upon contact with the analyte, thus cleaving the nucleic acid substrate to release a cleavage product comprising the detection moiety. The nucleic acid binding molecule binds to the cleavage product. USE - The biosensor is useful for detection of analyte in sample (claimed). ADVANTAGE - The method creates a simple sensor for the detection of an analyte that does not require enzymatic amplification. The biosensor for detecting analyte does not require enzymatic amplification such as rolling circle amplification (RCA), thus reducing the cost and complexity of the biosensor and the associated methods of use. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: (1) detection of at least one analyte in a sample, which involves: (a) placing the biosensor into a test solution comprising the sample; (b) retrieving the second support from the test solution; and (c) detecting the presence of the at least one analyte on the second support; and (2) a kit for detecting an analyte, comprising the biosensor, and instructions for use of the kit for detecting analyte.