▎ 摘　　要

NOVELTY - Detecting cauliflower mosaic virus 35S promoter using graphene and quantum dots fluorescence resonance energy transfer sensor involves preparing multi-walled carbon nanotubes/graphene nanotubes, nitrogen and sulfur isomorphous graphene quantum dots solution, and nitrogen and sulfur graphene quantum dot-capture probe dispersion, ultrasonic dispersing nitrogen and sulfur isomorphous graphene quantum dots solution in 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, oscillating, adding N-hydroxysuccinimide solution, oscillating, adding capture probe stock solution, and reacting. USE - Method for detecting cauliflower mosaic virus 35S promoter using graphene and quantum dots fluorescence resonance energy transfer sensor (claimed). ADVANTAGE - The method is simple, flexible, and fast. DETAILED DESCRIPTION - Detecting cauliflower mosaic virus 35S promoter using graphene and quantum dots fluorescence resonance energy transfer sensor comprises preparing multi-walled carbon nanotubes/graphene nanotubes, nitrogen and sulfur isomorphous graphene quantum dots solution, and nitrogen and sulfur graphene quantum dot-capture probe dispersion, ultrasonic dispersing nitrogen and sulfur isomorphous graphene quantum dots solution in 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, oscillating, adding N-hydroxysuccinimide solution, oscillating, adding capture probe stock solution, reacting, washing with ethanol, centrifuging, dispersing in tris-hydrochloric acid buffer, standing, constructing fluorescence resonance energy transfer transducer, adding prepared dispersion, ultrasonic dispersing, standing, determining fluorescence intensity, establishing standard curve, adding multi-walled carbon nanotubes/graphene nanotubes to dispersion, dispersing, standing, determining fluorescence intensity, adding different concentrations of target DNA, ultrasonic treating, standing, measuring fluorescence recovery, constructing standard curve, detecting transgenic soybeans, extracting using DNA extraction kit, determining fluorescence recovery intensity, and comparing to standard curve.