▎ 摘　　要

NOVELTY - Forming a substrate for detection of an analyte molecule in a sample involves providing a base layer, providing a binding layer on the base layer, depositing a top layer on the binding layer, patterning the top layer to expose portions of the binding layer, the exposed portions corresponding to multiple binding sites on the binding layer and providing a supramolecular structure associated with each binding site of multiple binding sites. The supramolecular structure comprises a core structure comprising multiple core molecules a capture molecule linked to the core structure at a first location, and a detector molecule linked to the core structure at a second location. USE - Method for forming a substrate for detection of an analyte molecule in a sample. ADVANTAGE - None given. DETAILED DESCRIPTION - Forming a substrate for detection of an analyte molecule in a sample involves providing a base layer, providing a binding layer on the base layer, depositing a top layer on the binding layer, patterning the top layer to expose portions of the binding layer, the exposed portions corresponding to multiple binding sites on the binding layer and providing a supramolecular structure associated with each binding site of multiple binding sites. The supramolecular structure comprises a core structure comprising multiple core molecules a capture molecule linked to the core structure at a first location, and a detector molecule linked to the core structure at a second location. The supramolecular structure is in an unstable state, such that the detector molecule is configured to be unbound from the core structure through cleavage of a link at the second location and such that the respective capture molecule and detector molecule spaced apart are at a pre-determined distance in the unstable state and where, in a stable state, the detector molecule and the capture molecule are linked together through binding to the analyte molecule, thus forming a link between the detector molecule and capture molecule, where the detector molecule remains linked to the core structure through the link with the capture molecule and such that the analyte molecule is associated with the individual binding site.