▎ 摘　　要

NOVELTY - Preparation of graphene oxide dual-target drug carrier material, comprises: step one: preparation of carboxymethylation graphene oxide; step two: preparation of graphene oxide-biological targeted molecule composition; and step three: preparation of graphene oxide dual-target drug carrier material. USE - The method is useful for preparing graphene oxide dual-target drug carrier material (claimed). ADVANTAGE - The method is for efficiently preparing carrier material and loading drug, which solves the problem of complex structure of nano drug carrier, high synthesis cost, low efficiency and difficult to prepare in mass production. DETAILED DESCRIPTION - Preparation of graphene oxide dual-target drug carrier material, comprises: step one: preparation of carboxymethylation graphene oxide: dispersing 0.5-5.0 g of graphite oxide in 100-1000 ml of 0.2 mol/L of sodium hydroxide (NaOH) solution, dispersing with ultrasound for 10-60 minutes; then adding 2-20 g of chloroacetic acid to the solution, stirring to react for 2-10 hours; centrifuging for 10-30 minutes with the speed of 16000 rpm, washing with water until the pH value is 7-8, the vacuumizing to obtain the carboxymethylation graphene oxide (GO-COOH); step two: preparation of graphene oxide-biological targeted molecule composition: dispersing 80-8000 mg of GO-COOH in 0.2-2 L of 40 mmol/L of 2-(N-morpholine) ethanesulfonic acid (MES) buffer solution, stirring and adding 0.4-4 g of N-hydroxyl succinamide (NHS) and dispersing with ultrasound for 10-30 minutes, then stirring and adding 0.2-2 g of 1-(3- dimethylamino propyl)-3-ethyl carbon dimethylamine (EDC) and dispersing with ultrasound for 10-30 minutes; the adding 0.1-1 g of biological targeted molecule to the solution above and dispersing with ultrasound for 10-30 minutes; then stirring the mixed solution for 6-12 hours; centrifuging for 10-30 minutes with the speed of 16000 rpm, washing with water until the pH is 7-8, then vacuumizing to obtain the graphene oxide-biological targeted molecule composition; step three: preparation of graphene oxide dual-target drug carrier material: dispersing 40-400 mg of graphene oxide biological targeted molecule composition in 200-2000 ml of NaOH solution with pH value of 12, dispersing for 2-4 hours with ultrasound; dialyzing the solution above with bag dialysis with cut off molecular weight of 7000 to neutral, concentrating the solution to 20-200ml; dissolving 100-1000 mg of germanium chloride hexahydrate (GeCl3.6H2O) and 200-2000 mg of iron chloride tetrahydrate (FeCl.4H2O) in 5-50 ml of water under the atmosphere of nitrogen, mixing and reacting for 12-24 hours; centrifuging for 10-30 minutes with the temperature of 16000 rpm to separate out the solid, washing with water to remove residual salt; dispersing the solid in 25-250 ml of water, dropping 4-40 ml of NaOH with concentration of 3 mol/L to the solution under the atmosphere of nitrogen (N2), reacting for 2-4 hours with the temperature of 65 degrees C; centrifuging the product with the speed of 16000 rpm, then vacuumizing the solid obtained. An INDEPENDENT CLAIM is a carrier material prepared by the preparation method of graphene oxide dual-target drug carrier material, the loaded drug is one or more of doxorubicin hydrochloride, paclitaxel, hydroxycamptothecine and daunomycin.