▎ 摘　　要

NOVELTY - Preparing phosphine-based ionic liquid modified nanocomposite involves dispersing first base material in anhydrous toluene, adding 3-chloropropyl triethoxysilane, stirring continuously, heating under nitrogen (N2) atmosphere to obtain the nanomaterial modified by chloropropyl group, namely A-CP, washing, drying, where the first base material is nano-silica (nSiO2), magnetic core-shell nano-silica (Fe3O4 doped nSiO2) or mesoporous silica-coated graphene (G doped mSiO2), dissolving polyethyleneimine (PEI) in absolute ethanol, stirring uniformly, adding the obtained material A-CP to the solution, stirring, heating, making PEI grafted on the surface of the obtained A-CP to obtain material A doped PEI, dispersing the obtained material A doped PEI in anhydrous toluene. USE - Method for preparing phosphine-based ionic liquid modified nanocomposite that is used for enriching phosphorylated peptide (claimed). ADVANTAGE - The prepared phosphine-based ionic liquid modified nanocomposite has larger specific surface area, excellent hydrophilicity, more metal solid loading capacity, excellent sensitivity, high specific selectivity, size exclusion effect and excellent reusability. The nano composite material is suitable for enrichment and purification of phosphorylated peptides of complex biological samples. The material has wide application prospect in the fields of proteomics and biomedicine. DETAILED DESCRIPTION - Preparing phosphine-based ionic liquid modified nanocomposite involves dispersing first base material in anhydrous toluene, adding 3-chloropropyl triethoxysilane, stirring continuously, heating under nitrogen (N2) atmosphere to obtain the nanomaterial modified by chloropropyl group, namely A-CP, washing, drying, where the first base material is nano-silica (nSiO2), magnetic core-shell nano-silica (Fe3O4 doped nSiO2) or mesoporous silica-coated graphene (G doped mSiO2), dissolving polyethyleneimine (PEI) in absolute ethanol, stirring uniformly, adding the obtained material A-CP to the solution, stirring, heating, making PEI grafted on the surface of the obtained A-CP to obtain material A doped PEI, dispersing the obtained material A doped PEI in anhydrous toluene, adding excess bromoalkyl phosphate, stirring, heating for reaction, washing, drying to obtain a phosphine-based functionalized ionic liquid modified material, namely A doped PEI-PFIL, where the bromoalkyl phosphate is diethyl (3-bromopropyl) phosphate, diethyl (4-bromobutyl) phosphate, and diethyl (5-bromopentyl) phosphate, dispersing the obtained material A doped PEI-PFIL in the concentrated hydrobromic acid diluted twice with deionized water, stirring, heating, neutralizing with sodium hydroxide solution, drying to obtain a material, dispersing the obtained material in a metal salt solution, reacting at 37degrees Celsius for 2 hours to obtain an affinity material for immobilizing metal ion, washing and drying to obtain nanomaterial A doped PEI-PFIL-Mn+, where Mn+= titanium ion (Ti4+), gallium ion (Ga3+), and the metal salt solution is titanium disulfate or gallium chloride. DESCRIPTION OF DRAWING(S) - The drawing shows a synthesis process of nanocomposite A doped PEI-PFIL-Mn+ (where, A=nSiO2, Fe3O4 doped nSiO2 or G doped mSiO2), PFIL=by diethyl (3-bromopropyl) phosphate, diethyl (4-bromobutyl) phosphate or diethyl (5-bromopentyl) phosphate, Mn+=Ti4+, Ga3+). (Drawing includes non-English language text).