• 专利标题:   Detecting mercury ion by using oligonucleotides and graphene oxide, involves adding oligonucleotides fluorescence probe marked with dye in buffer solution and adding graphene oxide to solution.
  • 专利号:   CN101887020-A
  • 发明人:   HUANG W, FAN Q, LIU X, MIAO L
  • 专利权人:   UNIV NANJING POSTS TELECOM
  • 国际专利分类:   G01N021/64
  • 专利详细信息:   CN101887020-A 17 Nov 2010 G01N-021/64 201119 Pages: 7 Chinese
  • 申请详细信息:   CN101887020-A CN10223065 09 Jul 2010
  • 优先权号:   CN10223065

▎ 摘  要

NOVELTY - Mercury ion detection involves adding oligonucleotides fluorescence probe marked with a dye in buffer solution. The fluorescence probe emits radiation of wavelength 480 nm. Graphene oxide i.e. a solid phase carrier is added to a solution. The solid phase carrier absorbs the oligonucleotides probe to generate oligonucleotides-graphene oxide compound. The mercury ion containing material reacts with oligonucleotides-graphene oxide compound to form a ring by connecting thymine-mercury ion. USE - Method for detecting mercury ion by using oligonucleotides and graphene oxide (claimed). DETAILED DESCRIPTION - Mercury ion detection involves adding oligonucleotides fluorescence probe marked with a dye in buffer solution. The fluorescence probe emits radiation of wavelength 480 nm. Graphene oxide i.e. a solid phase carrier is added to a solution. The solid phase carrier absorbs the oligonucleotides probe to generate oligonucleotides-graphene oxide compound. The mercury ion containing material reacts with oligonucleotides-graphene oxide compound to form a ring by connecting thymine-mercury ion. The fluorescence signal of dye is monitored in order to carry out qualitative and quantitative detection of mercury ion.