▎ 摘　　要

NOVELTY - A high affinity graphene oxide-nano platinum composite based substrate for simulation of peroxidase for catalyzing 3,3',5,5'-tetramethylbenzidine hydrochloride and hydrogen peroxide is claimed. The graphene oxide-nano platinum composite based substrate has high affinity for 3,3',5,5'-tetramethylbenzidine hydrochloride with Michaelis constant of 0.04064 mmol/l and has high affinity for hydrogen peroxide with Michaelis constant of 10.19 mmol/L. USE - High affinity graphene oxide-nano platinum composite based substrate for simulation of peroxidase (claimed). ADVANTAGE - The method ensures production of environmentally-friendly high affinity graphene oxide-nano platinum composite based substrate with excellent reproducibility and high stability by simple process at low temperature, and prevents the need for surfactant. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for preparing high affinity graphene oxide-nano platinum composite based substrate, which involves adding aqueous solution of chloroplatinic acid to an aqueous solution of graphene oxide, stirring, adding sodium borohydride, magnetic stirring at room temperature for 4 hours, and drying.