▎ 摘　　要

NOVELTY - Producing electronically readable single-stranded nucleic acid sequences, comprises e.g. (1) partially double-stranded detection gate nucleic acid construct (DG) comprises single-stranded detection substrate nucleic acid sequence characterized with partial region that is complementary with partial region of the reporter nucleic acid sequence (RSS), and toehold sequence (THS) region which is non-complementary to the RSS but is partially complementary to partial sequence of the single-stranded invader nucleic acid sequence (ISS), and (b) single-stranded RSS characterized with region that is complementary with partial region of the DSSS, and region which is complimentary to the pre-electronic readable regions of the signal substrate strand (SSS) but is non-complementary to either RSS, the DSSS or the ISS, and (2) reacting together the DG with the ISS in manner and under conditions so that the THS region on the DG initiates binding of the ISS and results in the displacement of the RSS. USE - The method is useful for producing electronically readable single-stranded nucleic acid sequences using biosensors for detecting single-stranded nucleic acids (claimed). ADVANTAGE - The method increases sensitivity of nucleic acid sequences. DETAILED DESCRIPTION - Producing electronically readable single-stranded nucleic acid sequences, comprises: (1) (ia) a partially double-stranded detection gate nucleic acid construct (DG) which comprises of (a) a single-stranded detection substrate nucleic acid sequence (DSSS) characterized with a partial region that is complementary with a partial region of the reporter nucleic acid sequence (RSS), and a toehold sequence (THS) region which is non-complementary to the RSS but is partially complementary to a partial sequence of the single-stranded invader nucleic acid sequence (ISS), and (b) a single-stranded RSS characterized with a region that is complementary with a partial region of the DSSS, and a region which is complimentary to the one or more pre-electronic readable regions of the signal substrate strand (SSS) but is non-complementary to either the RSS, the DSSS or the ISS, (iia) a single-stranded invader nucleic acid sequence (ISS) characterized with a region which is complementary to the THS of DSSS, (iii) a partially double-stranded signal gate construct (SG) comprising of (a0 the SSS characterized by being the complement of the RSS, including a toehold sequence (THS) also complementary to the RSS, and (b) multiple pre-electronically readable signal oligonucleotides (SO) characterized by being complementary to part of the SSS but not the THS of SSS; and (2) reacting together the DG with the ISS in a manner and under conditions so that the THS region on the DG initiates binding of the ISS and results in the displacement of the RSS, where the RSS further binds to the THS of SG and liberates the electronically readable single-stranded SO sequences. An INDEPENDENT CLAIM is also included for biosensor device comprising a graphene surface, where the surface is used to detect a change in charge associated with a method.