▎ 摘　　要

NOVELTY - Preparation of an electrochemical sensor involves preparing black nitrogen-doped reduced graphene oxide material (N-rGO), preparing nitrogen-doped reduced graphene oxide/poly(3,4-ethylenedioxythiophene) composite material (N-rGO-PEDOT), preparing gold nanocage dispersion (AuNC), polishing the glassy carbon electrode with alumina into a mirror surface, then ultrasonically washing it with distilled water and absolute ethanol, drying it, and obtaining the treated glassy carbon electrode for use, ultrasonically dispersing N-rGO-PEDOT material in methanol to obtain the N-rGO-PEDOT material mixture, dripping N-rGO-PEDOT material mixture to the surface of the treated glassy carbon electrode, drying naturally, rinsing with distilled water, and dripping AuNCs material to obtain N-rGO-PEDOT-AuNCs/GCE electrochemical sensor. USE - Preparation of electrochemical sensor used for simultaneous detection of rutin and xanthoamide in Zanthoxylum bungeanum (claimed). Uses include but are not limited to volatile oil, phenols, amides, proteins, unsaturated fatty acid and other nutrient substances and biological active ingredients. ADVANTAGE - The method is simple, low in cost, and enables preparation of electrochemical sensor with excellent repeatability, stability, detection accuracy, and is portable and suitable for large-scale accuracy detection of Zanthoxylum bungeanum. The sensor has strong anti-interference, high detection signal intensity, and has accurate detection result. DETAILED DESCRIPTION - Preparation of an electrochemical sensor involves dissolving graphene oxide and nitrogen doping agent in deionized water, transferring to a hydrothermal reactor, suction filtering to obtain black nitrogen-doped reduced graphene oxide material (N-rGO), washing, and freeze-drying, adding the N-rGO material and 3,4-ethylenedioxythiophene to acetonitrile, dripping anhydrous ferric chloride solution after ultrasonically-treating for preset time, stirring at room temperature for preset time, washing resulting product several times by centrifugation with distilled water and methanol, precipitating by centrifugation, drying to obtain nitrogen-doped reduced graphene oxide/poly(3,4-ethylenedioxythiophene) composite material (N-rGO-PEDOT), taking ethylene glycol into nitrogen, heating to reflux, then adding sodium sulfide solution, refluxing for preset time, adding polyvinylpyrrolidone solution, constant temperature treating for preset time, adding silver nitrate solution, reacting until the color (grass green) is stable, cooling to room temperature with ice water, washing the centrifuged product with acetone and distilled water in turn, dissolving the product obtained by centrifugation in deionized water to obtain a silver cube dispersion, taking the polyvinyl pyrrolidone aqueous solution and the silver cube dispersion, mixing and heating to reflux under nitrogen, dripping chloroauric acid solution, heating to reflux and then cooling to room temperature with ice water, centrifuging, collecting the centrifuged sediment, washing it with saturated sodium chloride solution, then washing with distilled water, and finally centrifuging again to get the sediment dissolved in distilled water to obtain the gold nanocage dispersion (AuNC), polishing the glassy carbon electrode with alumina into a mirror surface, then ultrasonically washing it with distilled water and absolute ethanol, drying it, and obtaining the treated glassy carbon electrode for use, ultrasonically dispersing N-rGO-PEDOT material in methanol to obtain the N-rGO-PEDOT material mixture, dripping N-rGO-PEDOT material mixture to the surface of the treated glassy carbon electrode, drying naturally, rinsing with distilled water, and dripping AuNCs material to obtain N-rGO-PEDOT-AuNCs/GCE electrochemical sensor. An INDEPENDENT claim is included for use of the electrochemical sensor for simultaneous detection of rutin and xanthoamide in Zanthoxylum bungeanum, where the detection involves preparing the sample solution to be tested, taking the sample solution to be tested and adding it to the electrolyte to obtain the mixed solution to be tested, treating with a three-electrode system, and measuring the rutin and xanthanamide in the mixed solution for detection.