▎ 摘　　要

NOVELTY - Performing multi-drug-resistant bacterial infection of burn wounds involves researching on the target bacteria effect of functionalized graphene nanocomposites modified by free radical initiators under in vivo and in vitro conditions. The nanomaterials target to bind to bacteria in vitro, and to the site of infection in vivo. The photothermal-induced targeted antibacterial effect of functionalized graphene nanocomposites modified by free radical initiators is studied under in vivo and in vitro conditions and using standard plate counting method and live/dead bacterial staining method to detect the in vitro targeted antibacterial ability of nanomaterials. USE - Method for performing multi-drug-resistant bacterial infection of burn wounds. ADVANTAGE - The method enables to perform multi-drug-resistant bacterial infection of burn wounds, which provides new treatment directions and strategies for multi-drug-resistant bacterial infections on burn wounds, and facilitates further exploration for multi-drug resistance of a new multifunctional nano platform for bacterial treatment. DETAILED DESCRIPTION - A mouse model of subcutaneous abscess of multidrug-resistant bacteria and trauma infection model is established, and observing the targeted antibacterial effect of nanomaterials induced by light and heat after injecting nanomaterials into the vein, and conduct quantitative analysis of colony count of abscess and infected tissue; and carrying out staining analysis of abscess tissue healing. The effect of functionalized graphene nanocomposite modified by free radical initiator on wound healing of multidrug-resistant bacteria infection and its mechanism of action is detected using electron spin resonance technology to detect the generation of alkane free radicals in nanomaterials, then using electron spin Resonance technology detects the generation of alkane radicals after nanomaterials are combined with bacteria, and electron spin resonance technology is used to detect the therapeutic mechanism of nanomaterials under normal oxygen and anaerobic conditions. The reactive oxygen under normal oxygen and anaerobic conditions Level detection, glutathione level detection and DNA damage detection, to explore the molecular biological effects of alkane free radicals in the oxidative stress response of bacteria, and immunohistochemistry and western blotting are used to explore the expression changes of wound healing related molecules and Cell signal transduction pathways closely related to this process. The research on the biosafety of functionalized graphene nanocomposites modified by free radical initiators is carried out after the nanomaterials co-cultured with 3T3 fibroblasts for 24 hours, and the proliferation of 3T3 fibroblasts are detected by the CCK-8 method, then passing hemolysis test detects the blood compatibility of the nanomaterials and 30 days after the injection of the nanomaterials through the vein of the mice, observing whether there are pathological changes in the main organs and blood systems of the mice, and explore the acute and chronic toxicity effect.