▎ 摘　　要

NOVELTY - Preparation of platinum-copper-graphitic carbon nitride/reduced graphene oxide-labeled electrochemical immunosensor involves polishing a glassy carbon electrode, cleaning, dripping nano-gold thionine-modified graphene, rinsing, drying, dripping tumor marker-capturing antibody (Ab1), rinsing, drying, adding bovine serum albumin solution, blocking the non-specific active site on the surface, dripping tumor marker antigen (Ag) solution, rinsing, drying, dripping platinum-copper-graphitic carbon nitride/reduced graphene oxide-detecting antibody (Ab2) heat-preservation solution, and drying. USE - Preparation of platinum-copper-graphitic carbon nitride/reduced graphene oxide-labeled electrochemical immunosensor used for detecting tumor marker e.g. CA724, CA242 and carcinoembryonic antigen (CEA) (all claimed). ADVANTAGE - The method enables preparation of platinum-copper-graphitic carbon nitride/reduced graphene oxide-labeled electrochemical immunosensor, which effectively detects tumor marker with excellent specificity, high sensitivity and low detection limit within short period of time. DETAILED DESCRIPTION - Preparation of platinum-copper-graphitic carbon nitride/reduced graphene oxide-labeled electrochemical immunosensor involves polishing a glassy carbon electrode having diameter of 4 mm with aluminum oxide polishing powder, cleaning the electrode surface with ultrapure water, dripping 6 mu L, 1.0-2.0 mg/mL nano-gold thionine-modified graphene onto the electrode surface, drying at room temperature, rinsing the electrode surface with ultrapure water, drying, dripping 6 mu L, 8.0-12.0 mu g/mL tumor marker-capturing antibody (Ab1) to the electrode surface, rinsing with ultrapure water, drying in a refrigerator at 4 degrees C, adding 3 mu L, 1.0-3.0 mg/mL bovine serum albumin solution to the electrode surface, blocking the non-specific active sites on the electrode surface, rinsing the electrode surface with ultrapure water, drying in the refrigerator at 4 degrees C, dripping 6 mu L, 1 fg/mL to 10 ng/mL tumor marker antigen (Ag) solution, rinsing the electrode surface with ultrapure water, drying in the refrigerator at 4 degrees C, dripping 6 mu L 2.0-3.0 mg/mL platinum-copper-graphitic carbon nitride/reduced graphene oxide-antibody (Ab2) detecting heat-preservation solution, placing in a refrigerator at 4 degrees C and drying.