▎ 摘　　要

NOVELTY - Method for producing biotinylated antibody sensor based on genetic recombinant phycocyanin membrane attack complex (MAC) and graphene oxide quantum dots, involves transferring expression plasmid pMAC into Escherichia coli, inoculating into Luria-Bertani (LB) medium, culturing, transferring the saturated bacterial solution to LB medium containing spectinomycin, adding isopropyl thiogalactoside, ultrasonically crushing, centrifuging, recovering supernatant, purifying by affinity chromatography column, obtaining purified protein MAC, configuring to protein MAC solution, modifying graphene oxide quantum dots by chitosan, arranging the graphene oxide quantum dot-chitosan complex into a graphene oxide quantum dot-chitosan complex solution, preparing a biotinylated antibody solution, adding the biotinylated antibody solution to the protein MAC solution, incubating, adding the graphene oxide quantum dot-chitosan complex solution, incubating, and exciting by laser. USE - The method is useful for producing biotinylated antibody sensor based on genetic recombinant phycocyanin MAC and graphene oxide quantum dots (claimed). ADVANTAGE - The produced sensor has high sensitivity. DETAILED DESCRIPTION - Method for producing biotinylated antibody sensor based on genetic recombinant phycocyanin membrane attack complex (MAC) and graphene oxide quantum dots, involves cloning maltose binding protein gene, histidine tag, phycocyanin apoprotein subunit gene, core streptavidin gene, and phycocyanin cleavage isomerase E and F coding genes under same promoter, cloning heme oxygenase 1 gene and phycocyanin ferredoxin reductase gene under another promoter, constructing an expression plasmid pMAC, transferring the expression plasmid pMAC into Escherichia coli, obtaining engineering strain BMAC, inoculating into Luria-Bertani (LB) medium, culturing overnight to form a saturated bacterial solution, transferring the saturated bacterial solution to LB medium containing spectinomycin, adding isopropyl beta -D-1-thiogalactopyranoside (IPTG) inducer, avoiding light-induced expression, collecting bacterial liquid, ultrasonically crushing under an ice bath, centrifuging, recovering supernatant, purifying by affinity chromatography column, obtaining purified protein MAC, configuring the protein MAC to a protein MAC solution, modifying graphene oxide quantum dots by chitosan, obtaining a graphene oxide quantum dot-chitosan complex, arranging the graphene oxide quantum dot-chitosan complex into a graphene oxide quantum dot-chitosan complex solution, preparing a biotinylated antibody solution, adding the biotinylated antibody solution to the protein MAC solution, incubating at room temperature, adding the graphene oxide quantum dot-chitosan complex solution, incubating at room temperature, and exciting by laser.