▎ 摘　　要

NOVELTY - Analyzing nucleic acid loading rate of graphene oxide comprises (1) drawing a fluorescent signal standard curve of the nucleic acid, configuring a plurality of nucleic acid solutions to be tested according to the concentration step distribution, the nucleic acid solution to be tested comprises DNA and/or RNA and modified by a fluorescent group, and detecting by a fluorescence spectrophotometer signal value to obtain a standard fluorescent signal value of the nucleic acid solution to be tested at each concentration, and drawing a fluorescence signal standard curve based on the concentration coordinate, (2) using graphene oxide quenches the fluorescence, and the nucleic acid solution to be tested is sufficiently mixed with a known concentration of the graphene oxide solution to obtain a mixed solution, after the nucleic acid is loaded to the graphene oxide, the nucleic acid fluorescence may occur due to the graphene oxide and quenching. USE - The method is useful for analyzing nucleic acid loading rate of graphene oxide. DETAILED DESCRIPTION - Analyzing nucleic acid loading rate of graphene oxide comprises (1) drawing a fluorescent signal standard curve of the nucleic acid, configuring a plurality of nucleic acid solutions to be tested according to the concentration step distribution, the nucleic acid solution to be tested comprises DNA and/or RNA and modified by a fluorescent group, and detecting by a fluorescence spectrophotometer signal value to obtain a standard fluorescent signal value of the nucleic acid solution to be tested at each concentration, and drawing a fluorescence signal standard curve based on the concentration coordinate, (2) using graphene oxide quenches the fluorescence, and the nucleic acid solution to be tested is sufficiently mixed with a known concentration of the graphene oxide solution to obtain a mixed solution, after the nucleic acid is loaded to the graphene oxide, the nucleic acid fluorescence may occur due to the graphene oxide, quenching, causing the fluorescence value of the mixed solution to decrease and detecting the fluorescence signal value of the nucleic acid solution after the fluorescence quenching of the graphene oxide by a fluorescence spectrophotometer, calculating the concentration of the nucleic acid in the solution at the current time using the standard curve of the fluorescent signal, (3) calculating the loading ratio of graphene oxide to nucleic acid, and the loading ratio L is the ratio of the mass of the supported nucleic acid to the mass of graphene oxide, and calculate the formula: L = (C1-C2) V/C GOV x % = (C1-C2)/CGOx %=(n1-n2) M/C GO x %, where C1 and C2 are the mass concentrations of the nucleic acids in the solution before and after the loading, respectively, and CGO is the GO in the solution. The mass concentration, V is the solution volume, n 1 and n 2 are the molar concentration of the nucleic acid in the solution before and after the loading, respectively, and M is the molar mass of the nucleic acid.