▎ 摘　　要

NOVELTY - Aptamer fluorescent brain natriuretic peptide detection device has a detection control mechanism, a fluorescence fast detection mechanism, and aptamer fluorescent sensor. The detection control mechanism comprises a power supply module (101), a microcontroller (102) excitation light source (103), a signal collecting and photoelectric conversion module, a signal chain processing module, a Bluetooth module. The intelligent mobile phone outputs the power supply voltage to power supply module. The power supply is realized for the detection control mechanism. The signal chain processing module is used for receiving microcontroller signal of the transmission and performing analog-to-digital conversion to electric signal, and transmitting the processing result to the microcontroller. The inner shell is fixedly installed with the signal collecting and photoelectric conversion module (104) and a main control circuit board and is provided with an excitation light source fixing hole. USE - Aptamer fluorescent brain natriuretic peptide detection device based on smart phone. ADVANTAGE - The device fills the blank of portable digital brain natriuretic peptide fluorescence detection and the brain natriuretic peptide fast detection requirement, and has a good development prospect in the field of high sensitivity and high efficiency instant detection. DETAILED DESCRIPTION - Aptamer fluorescent brain natriuretic peptide detection device has a detection control mechanism, a fluorescence fast detection mechanism, and aptamer fluorescent sensor. The detection control mechanism comprises a power supply module, a microcontroller excitation light source, a signal collecting and photoelectric conversion module, a signal chain processing module, a Bluetooth module. The input end of the power supply module is used for connecting with the Universal Serial Bus (USB) interface of the intelligent mobile phone. The intelligent mobile phone outputs the power supply voltage to the power supply module. The power supply is realized for the detection control mechanism. The microcontroller and the excitation light source, the signal collecting and photoelectric conversion module, the signal chain processing module are signal connected, and connected with the intelligent mobile phone signal through the Bluetooth module. The excitation light source is used for according to the control signal of the microcontroller turn on generating excitation light of set wavelength. The signal collecting and photoelectric conversion module is used for capturing the fluorescent signal generated by the solution to be tested according to the control signal of the microcontroller and converted the fluorescent signal into electric signal and transmitting to the microcontroller. The signal chain processing module is used for receiving the microcontroller signal of the transmission and performing analog-to-digital conversion to the electric signal, filtering and amplifying the processing, and transmitting the processing result to the microcontroller. The Bluetooth module is used for transmitting the instruction signal of the intelligent mobile phone to the microcontroller and transmitting the obtained microcontroller result to the intelligent mobile phone to display. The fluorescent fast detection mechanism is used for detecting brain natriuretic peptide under the control of the detection control mechanism of the aptamer fluorescent sensor. The fluorescent fast detecting mechanism comprises an intelligent mobile phone bracket and a main box. The intelligent mobile phone bracket is used for placing the intelligent mobile phone. The intelligent mobile phone is used for supplying power, the detecting operation and result display. The main box is provided with an inner shell, and the inner shell and the main box have a distance. The inner shell is fixedly installed with the signal collecting and photoelectric conversion module and a main control circuit board and is provided with an excitation light source fixing hole. The main control circuit board is integrated with the power supply module, the microcontroller, the signal chain processing module and the Bluetooth module. The inner portion of the inner shell is provided with a fluorescent signal reaction table. The portion of the fluorescent signal reaction table far away from the excitation light source fixing hole is provided with a fluorescent signal reaction tank. The excitation light source fixing hole are coaxially arranged to LED the excitation light source with the excitation light source fixing hole. An excitation filter groove is arranged at the joint of the excitation light screw thread fixing hole and the fluorescence signal reaction tank. The excitation filter groove is used for loading the excitation filter. The connecting body is provided with emitting light receiving hole. The emitting light receiving hole is connected with the fluorescent signal reaction tank. The emitting light receiving hole and the excitation light screw thread hole are horizontally vertical to the node of the fluorescent signal reaction tank. An INDEPENDENT CLAIM is included for a sensing method based on the aptamer fluorescent brain natriuretic peptide detection device, which involves: (a) mixing the 100nmol/l nucleic acid aptamer solution and 40 ml carboxylated graphene dispersion liquid, controlling the solution pH value environment at 7.2-7.4, room temperature standing for 20±5 minutes; (b) adding the solution to be tested with a certain concentration of brain natriuretic peptide into the mixed solution, standing for 35±5 min at room temperature; (c) taking proper solution to be tested in the quartz cuvette, placing the quartz cuvette in the fluorescent signal reaction tank, and setting parameters on the smart mobile phone, where the excitation wavelength is 492nm, the emission wavelength is 519nm, and the emission spectrum detection range 505-610nm; and (d) detecting the mixed solution fluorescence intensity according to the linear response relation of the substance concentration and fluorescence intensity to obtain the brain natriuretic peptide concentration in the solution. DESCRIPTION OF DRAWING(S) - The drawing shows a schematic view of aptamer fluorescent brain natriuretic peptide detection device. 101Power supply module 102Microcontroller 103Excitation light source 104Signal collecting and photoelectric conversion module 105Signal chain processing module