▎ 摘　　要

NOVELTY - Preparing bioseparation medium involves mixing graphene oxide with deionized water and stirring to obtain mixed solution. The mixed solution is subjected to ultrasound treatment. The solution is cooled and atomized into aerosol droplets through quartz tube. Polytetrafluoroethylene membrane is collected and dried to obtain graphene microspheres. The obtained graphene microspheres are immersed in dye solution at 50-60 degrees C and heated for 30-50 minutes after adding sodium chloride solution. The mixture is oscillates for 30-40 minutes and temperature is raised to 65-80 degrees C. USE - Method for preparing bioseparation medium used in separation and purification of papaya enzymes. ADVANTAGE - The method is fast, simple and suitable for large-scale production, and has separation capacity, extraction activity and high purity. DETAILED DESCRIPTION - Preparing bioseparation medium involves mixing graphene oxide with deionized water and stirring to obtain mixed solution. The mixed solution is subjected to ultrasound treatment. The solution is cooled and atomized into aerosol droplets through quartz tube. Polytetrafluoroethylene membrane is collected and dried to obtain graphene microspheres. The obtained graphene microspheres are immersed in dye solution at 50-60 degrees C and heated for 30-50 minutes after adding sodium chloride solution. The mixture is oscillates for 30-40 minutes and temperature is raised to 65-80 degrees C. The mixture is added with sodium bicarbonate solution and oscillated for 4-5 hours. The obtained product is washed to obtain graphene bioseparation medium.