▎ 摘　　要

NOVELTY - Detecting anti-immunoglobulin G (Anti-IgG) concentration involves: (1) preparation of 3,5-dibromo salicylaldehyde Schiff base nickel complexes-graphene oxide electrochemical immunosensor; (2) binding the immunosensor with different concentrations of Anti-IgG and washing twice with water; and (3) processing the sensor as the working electrode, saturated calomel electrode as reference electrode, and molybdenum and gold electrodes as auxiliary electrode, using phosphate buffer saline (PBS) solution, taking cyclic volt-ampere scanning, and recording concentration of Anti-IgG solution. USE - For detecting anti-immunoglobulin G (Anti-IgG) concentration (claimed). ADVANTAGE - The method is simple and rapid, shows high sensitivity, stability and reproducibility. DETAILED DESCRIPTION - A method of detecting anti-immunoglobulin G (Anti-IgG) concentration involves: (1) preparation of 3,5-dibromo salicylaldehyde Schiff base nickel complexes-graphene oxide electrochemical immunosensor by: (a) reacting 10 mg of graphene oxide and 0.05 mmol of 3,5-dibromo salicylaldehyde Schiff base nickel complexes in DMF, in presence of N,N-dimethyl formamide, and ultrasonically dispersing for 2 hours to obtain 3,5-dibromo salicylaldehyde Schiff base nickel complexes-oxidized graphene suspension; (b) polishing gold electrodes with Al2O3 powder, and rinsing twice with water, and then ultrasonically cleaning with ethanol, and then carrying out infrared drying for 3 minutes; dripping the gold electrodes in to the 3,5-dibromo salicylaldehyde Schiff base nickel complexes-oxidized graphene suspension and infrared drying, to obtain 3,5-dibromo salicylaldehyde Schiff base nickel complexes-graphene oxide modified gold electrode as L1 electrodes; (c) dripping a mixture of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDAC) and hydroxysuccinimide (NHS) the L1 electrodes, and a horizontal placing for 2 hours to obtain an activated 3,5-dibromo salicylaldehyde Schiff base nickel complexes-graphene oxide modified gold electrode as L2 electrodes, and stored in refrigerator at 4 degrees C; (d) dripping the L2 electrodes in 10 mu l of h-IgG coating solution to obtain h-IgG 3,5-dibromo salicylaldehyde Schiff base nickel complexes-graphene oxide modified gold electrode as L3 electrodes and stored in refrigerator at 4 degrees C; (e) dripping the L3 electrodes in 10 mu l of bovine serum albumin solution (BSA) and infrared drying, to obtain 3,5-dibromo salicylaldehyde Schiff base nickel complexes-graphene oxide electrochemical immunosensor; (2) binding the immunosensor with different concentrations of Anti-IgG and washing twice with water; (3) processing the sensor as the working electrode and a saturated calomel electrode as the reference electrode, molybdenum and gold electrodes as auxiliary electrode, using phosphate buffer saline (PBS) solution, taking cyclic volt-ampere scanning, recording different concentration of Anti-IgG solution.