• 专利标题:   Preparing nano-copper/graphene modified electrode ginsenoside Rg1 molecular imprinting sensor comprises e.g. preparing nano-copper/graphene modified liquid, dispersing, placing under the infrared light, volatilizing and drying.
  • 专利号:   CN107219279-A, CN107219279-B
  • 发明人:   LI H, LI D, YANG Q
  • 专利权人:   UNIV JINAN
  • 国际专利分类:   G01N027/30
  • 专利详细信息:   CN107219279-A 29 Sep 2017 G01N-027/30 201781 Pages: 7 Chinese
  • 申请详细信息:   CN107219279-A CN10380724 25 May 2017
  • 优先权号:   CN10380724

▎ 摘  要

NOVELTY - Preparing nano-copper/graphene modified electrode ginsenoside Rg1 molecular imprinting sensor comprises e.g. (i) preparing nano-copper/graphene modified liquid, (ii) preparing nano-copper/graphene modified electrode, (iii) preparing ginsenoside Rg1 molecularly imprinted polymers, (iv) taking the specific amount of ginsenoside Rg1 molecularly imprinted polymer, dispersing in gelatin solution, taking ginsenoside Rg1 molecularly imprinted polymer solution, adding solution in a dropwise into the nano-copper/graphene-modified electrode, placing under the infrared light, volatilizing and drying. USE - The method is useful for preparing nano-copper/graphene modified electrode ginsenoside Rg1 molecular imprinting sensor (claimed). ADVANTAGE - The method: has high compatibility, high sensitivity, good specificity, rapid detection and can be utilizes repeatedly. DETAILED DESCRIPTION - Preparing nano-copper/graphene modified electrode ginsenoside Rg1 molecular imprinting sensor comprises (i) preparing nano-copper/graphene modified liquid: adding 80-86 wt.% water, 2-5 wt.% copper acetate, 6-12 wt.% graphene oxide, at room temperature ultrasound for 20 minutes, dispersing uniformly, adding 3-8 wt.% ascorbic acid, heating at 60 plus minus 2 degrees C, stirring and reacting for 60 minutes to obtain nano copper/graphene modified liquid, (ii) preparing nano-copper/graphene modified electrode: coating glassy carbon electrode is sequentially with 0.2 m, using 0.01 mu m polishing powder for surface polishing, using secondary distilled water for ultrasonic cleaning, washing with ethanol, drying, dropping 20-25 mu l nano copper/graphene modified solution on the surface of glassy carbon electrode, placing under the infrared lamp, evaporating of the dry solvent to obtain nano-copper/graphene modified electrode, (iii) preparing ginsenoside Rg1 molecularly imprinted polymers: adding 62-70 wt.% methanol, 6-12 wt.% dimethylphenylene glycol dimethacrylate, 4-8 wt.% itaconic acid, 7-16 wt.% 1-allyl-3-ethylimidazolium bromide, 1-3 wt.% azobisisobutyronitrile, 8-12 wt.% ginsenoside Rg1, stirring and dissolving, filling inert gas and oxygen for 15 minutes, anaerobic atmosphere, stirring and reacting at 60 plus minus 2 degrees C for 24-28 hours, immersing resulting product with in a mixed solution of methanol and acetic acid in a ratio of 8:1 for 12-15 hours, removing the template molecule and drying to obtain ginsenoside Rg1 molecularly imprinted polymers, (iv) preparing ginsenoside Rg1 molecular imprinting electrochemical sensor: taking the specific amount of ginsenoside Rg1 molecularly imprinted polymer, dispersingin 1% gelatin solution, taking 20 g/l ginsenoside Rg1 molecularly imprinted polymer solution, adding 12-15 mu l of the solution in a dropwise into the nano-copper/graphene-modified electrode obtained in the step (ii), placing under the infrared light, volatilizing and drying the solvent to obtain ginsenoside Rg1 molecular imprinting electrochemical sensor.