▎ 摘　　要

NOVELTY - Two-dimensional sub-nano channel detection system has a porous support and a functionalized graphene oxide film arranged on the porous support. The porous support body includes an anodized aluminum oxide film or a porous polymer film. The functionalized graphene oxide film is assembled from a set of sheet-like single-layer graphene oxide layers. The interlayer spacing is angstrom level. The interlayer spacing of all two adjacent single-layer graphene oxides constitutes sub-nanometer pores. The outer surface of the functionalized graphene oxide film is decorated with probe molecules that capture target molecules. The probe molecule interacts with the target molecule to be tested. The target molecule to be tested has its own electric field, which amplifies the ion current signal of the sub-nanometer channel of the two-dimensional sub-nanometer channel detection system and realizes high-sensitivity detection of the target molecule. USE - Two-dimensional sub-nano channel detection system. ADVANTAGE - The system distinguishes electrolytes of different sizes, overcomes the defect of high background signal of one-dimensional nanochannel, solves the problem of low detection sensitivity of target molecules, increases the detection limit to 1aM, has a simple preparation method and stable performance, and is convenient for nucleic acid molecule detection. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: (1) a nucleic acid molecular detection device, which comprises a symmetrical electrolytic cell and the two-dimensional sub-nanometer hole detection system, the graphene oxide thin film is sandwiched between the symmetrical electrolytic cells, and the symmetrical electrolytic cells are filled with electrolyte, and the electrolyte includes Lithium chloride (LiCl) and LiTf2N; and (2) a method for detecting nucleic acid molecules using the device, which involves: (a) dropping 30 μL target nucleic acid molecule solution, leaving to stand at room temperature for 12 hours, washing with deionized water for 3-5 minutes, and drying with nitrogen to obtain Functionalized graphene oxide film for capturing target nucleic acid molecules, preparing hybridization buffer solutions of nucleic acid molecules of different standard concentrations, preparing functionalized graphene oxide films captured by nucleic acid molecules of different standard concentrations, and preparing functionalized graphene oxide films for capturing nucleic acid molecules to be tested; (b) detecting the ionic current signal of the functionalized graphene oxide film modified by the probe; (c) taking the concentration of nucleic acid molecules as the abscissa and the current increment as the ordinate, performing curve fitting according to the logistic regression model, and drawing a standard curve; and (d) taking β nucleic acid molecule to be tested sample into the standard curve drawn to calculate the concentration of nucleic acid molecules in the sample to be tested.