▎ 摘　　要

NOVELTY - Microfluidic chip comprises oil phase channel, first flow path connected to the oily substance channel and formed to flow the oily substance. A second flow path connected to the water-phase material channel to flow the water-water material, and connected to the first branch flow path and the second branch flow path branched from the first flow channel to form a junction region. A third flow path connected to the junction region and arranged in a straight line with the second flow path and a fine droplet channel connected to a downstream of the third flow path, where the first branch flow path and the second branch flow path cross each other and are connected to the second flow path to a junction region in a vertical direction of the second flow path. An oily material supplied through the first branch flow path and the second branch flow path and an aqueous phase material supplied through the second flow path are introduced to form fine droplets. USE - Microfluidic chip used in tumor treatment studies. ADVANTAGE - The microfluidic chip easily control the size of the tumor spheroid and the number of cells, and are particularly suitable for the study of the photothermal treatment effect on tumor cells. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: (1) a method for analyzing the effect of photothermal treatment of tumor cells, which involves connecting first flow path to the oily substance channel and forming to flow the oily substance, connecting second flow path to the water-phase material channel to flow the water-water material, and being connected to the first branch flow path and the second branch flow path branched from the first flow channel to form a junction region, connecting third flow path connected to the junction region and arranging in a straight line with the second flow path, where the first branch flow path and the second branch flow path cross each other and connecting to the second flow path to a junction region in a vertical direction of the second flow path, introducing an oily material supplied through the first branch flow path and the second branch flow path and an aqueous phase material supplied through the second flow path to form microscopic droplets, and the formed microscopic droplets are third flow paths, preparing a microfluidic chip based on a microdroplet that moves to the microdroplet channel through, generating fine droplets by injecting a water phase substance including an oil phase substance and a tumor cell into the oil phase substance channel and the water phase substance channel, respectively, injecting and culturing nanoparticles exhibiting a photothermal effect into the micro droplets, and irradiating the cultured microdroplets with a laser and analyzing the survival and death of tumor cells contained in the microdroplets; and (2) a method for producing a three-dimensional tumor cell, which involves injecting an oily substance and an aqueous substance into the microfluidic chip based on the microdroplet to generate microdroplets.