▎ 摘　　要

NOVELTY - Preparing dual electric field drive sensor involves designing the shape of the circuit by using computer drawing software, cutting out the flexible polyimide substrate, and setting N conductive holes connected by lines at one end of the flexible polyimide substrate. A layer of uniform copper film is sprayed on the surface of the flexible polyimide substrate. A photolithography technology is utilized to coat the photoresist according to the pre-designed circuit shape coating on the copper film. The photoresist-coated flexible polyimide substrate is placed under ultraviolet light for exposure. The exposed flexible polyimide substrate utilizes chemical developer. The bovine serum albumin is continuously added dropwise to the surface of the three-electrode system in the reaction chamber for incubation after rinsing followed by rinsing the working electrode with distilled water after the incubation is completed to obtain a dual electric field driven sensor. USE - Method for preparing dual electric field drive sensor for detecting pesticide residues. ADVANTAGE - The method improves the binding efficiency, sensor sensitivity and detection efficiency, shortens the reaction time, prevents the generation of bubbles on the sensor surface due to excessive voltage, establishes a more effective electric field enhancement strategy, and achieves rapid and selective detection of pesticide residues in natural samples. DETAILED DESCRIPTION - Preparing dual electric field drive sensor involves designing the shape of the circuit by using computer drawing software, cutting out the flexible polyimide substrate, and setting N conductive holes connected by lines at one end of the flexible polyimide substrate. A layer of uniform copper film is sprayed on the surface of the flexible polyimide substrate. A photolithography technology is utilized to coat the photoresist according to the pre-designed circuit shape coating on the copper film. The photoresist-coated flexible polyimide substrate is placed under ultraviolet light for exposure. The exposed flexible polyimide substrate utilizes chemical developer. The unexposed area is dissolved after the development. The exposed area is leaved to form a copper foil circuit. The developed flexible polyimide substrate is immersed in the potassium cyanide potassium solution. The electroplating technique forms an unclosed circular gold film at the other end of the opposite conductive hole as the counter electrode. The open end of the circular gold film of the counter electrode is connected to the conductive holes. A working electrode is arranged in the inner area of the counter electrode. The arc-shaped Ag/AgCl slurry is coated on the blank annular area where the counter electrode is not closed and dried as a reference electrode. The counter electrode, the working electrode, and the reference electrode formed at this time are called a three-electrode system. The three-electrode system is all in the hollow area of the hollow reaction chamber and is not in contact with the hollow reaction chamber. A flexible printing substrate is obtained. Two groups of independent copper foil areas are electroplated on the back of the three-electrode system of the flexible printing substrate. Each copper foil area is composed of M independent copper foil sheets where M is a positive integer. The area distribution of each group of copper foil areas are adjusted by the copper foil sheet. The two groups of copper foil areas pass through the conductive holes of the flexible polyimide substrate. The dynamic bias voltage and peak-to-peak voltage are fixed. Each copper foil area is assigned a set of electric fields. The dual-channel signal generator is turned on during operation. The three electrodes are connected to the hollow area of the system reaction chamber which provides a set of complementary electric field environments. A graphene oxide solution is prepared. The graphene oxide solution is dropped on the surface of the three-electrode system in the reaction chamber. An electrodeposition is carried out at an electrochemical workstation and rinsed the working electrode with distilled water after deposition. A chloroauric acid solution is prepared and dropped the chloroauric acid solution on the surface of the three-electrode system in the reaction chamber. An electrodeposition is performed at an electrochemical workstation and rinsed the working electrode with distilled water after deposition. The surface of the three-electrode system added in the reaction chamber activates the working electrode. The working electrode is rinsed with distilled water. The pesticides are dripped onto the surface of the three-electrode system in the reaction chamber after rinsing. The antibody is incubated after the pesticide antibody incubation is completed. The working electrode is rinsed with distilled water. The bovine serum albumin is continuously added dropwise to the surface of the three-electrode system in the reaction chamber for incubation after rinsing followed by rinsing the working electrode with distilled water after the incubation is completed to obtain a dual electric field driven sensor.