▎ 摘　　要

NOVELTY - Oxidoreductase electrode comprises a substrate electrode, an electronic conductor, an electron mediator, an enzyme and a coenzyme. The electronic conductor comprises graphene oxide, reducing graphene oxide, carbon nano tube, poly-dopamine, nano-gold, metal organic framework (MOF), polyethylene imine and/or biomimetic mineralized titanium dioxide material. The coenzyme comprises at least one of NADH-IL or PEI-Fc-NADH. The coenzyme and the enzyme are fixed on the enzyme electrode. USE - The electrode is useful for enzyme electrocatalytic reduction (claimed). ADVANTAGE - The electrode: has high detection limit and high sensitivity, enzyme electrocatalysis required to be able to tolerate high concentration of substrate, high catalytic efficiency; realizes the in-situ regeneration of the coenzyme; improves the reaction efficiency; realizes the enzymatic electrocatalytic efficient preparation of chiral compounds and has good application prospects. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for: (1) preparing oxidoreductase electrode comprising mixing the immobilized mixture of enzyme and electronic conductor and electron mediator and coenzyme on the surface of the substrate electrode to obtain the oxidoreductase electrode; (2) an enzyme-electric reactor for enzyme electrocatalytic reduction, which uses three-electrode system, the oxidoreductase electrode as the working electrode, the reference electrode is saturated calomel electrode, hydrogen electrode, silver chloride electrode or mercury oxide electrode and the counter electrode is platinum wire electrode or carbon electrode; and (3) carrying out catalytic reduction reaction by using the enzyme reactor, which is carried out in cyclic voltammetry and/or timing current method, the potential scanning rate of the cyclic voltammetry is 500 mV/second, adding 1-20 mg coenzyme NADH buffer solution, introducing nitrogen before the reaction, where the reaction substrate is the substrate ketone acid of amino acid dehydrogenase, substrate ketone of amine dehydrogenase and substrate ketone of alcohol dehydrogenase, the concentration range of the substrate is 5-500 mM, the buffer solution is at least one of phosphate buffer solution, borate buffer solution, citrate buffer solution, carbonate buffer solution and Tris-HCl buffer solution.