▎ 摘　　要

NOVELTY - Preparing polypyrrole conductive neural scaffold comprises (i) dissolving polylactic acid in hexafluoro-2-propanol to prepare spinning solution, electrically spinning under electrostatic field, preparing nanofiber film on indium tin oxide-plated polyester conductive film and drying to obtain polylactic acid electrostatic spinning film-A; (ii) ultrasonically dispersing graphene or its derivatives in ultra-pure water, and adding sodium dodecyl benzene sulfonate and pyrrole to obtain electro-deposition solution-B; (iii) using fiber film-A which uses indium tin oxide-plated polyester conductive film as carrier was used as anode and using platinum sheet as cathode, immersing in deposition solution-B, applying direct current power between two electrodes, electro-chemically depositing, and cleaning and drying to obtain composite film-C; (iv) preparing dopamine solution-D using trisaminomethane buffer solution; and (v) soaking composite film-C in dopamine solution-D, washing and drying. USE - The method is useful for preparing polypyrrole conductive neural scaffold. ADVANTAGE - The method is simple, and easy to operate and control; and has mild reaction condition. The neural scaffold has good electrical conductivity and biocompatibility, and potential clinic application value in peripheral nerve repair field. DETAILED DESCRIPTION - Preparing polypyrrole conductive neural scaffold comprises (i) dissolving 5-15 wt.% polylactic acid in hexafluoro-2-propanol to prepare spinning solution, electrically spinning at 8-15x 105 V/m for 10-100 minutes under electrostatic field, preparing nanofiber film on indium tin oxide-plated polyester conductive film and drying at room temperature in vacuum drying box to obtain polylactic acid electrostatic spinning film-A; (ii) ultrasonically dispersing 0.01-0.15 wt.% graphene or its derivatives in ultra-pure water, and adding 5-15 mM sodium dodecyl benzene sulfonate and 40-200 mM pyrrole at 4 degrees C to obtain electro-deposition solution-B; (iii) using fiber film-A which uses indium tin oxide-plated polyester conductive film as carrier was used as anode and using platinum sheet as cathode, immersing deposition solution-B, applying 0.5-5 mA/cm direct current power between two electrodes, electrically depositing for 10-60 minutes, and cleaning and drying to obtain graphene or its derivatives/polypyrrole composite film-C; (iv) preparing 1-3.3 mg/ml dopamine solution-D using trisaminomethane buffer solution with pH 8-11; and (v) soaking composite film-C in dopamine solution-D for 1-5 hours, washing and drying.