▎ 摘　　要

NOVELTY - Preparing tissue-induced nanofibrous biomaterial with graphene quantum dot tweezers trapping host cell homing involves putting 5-10 g citric acid powder into a microwave oven, pyrolyzing for 80-90 minutes under 1000 watt power to obtain dark brown liquid, pouring into 10-20 ml of 1 mol/l sodium hydroxide (NaOH) solution, stirring at 4℃ for 30 minutes to obtain a graphene quantum dots (GQDs) solution, dissolving 100-200 mmol CXCL-8, 8.717 µmol -17.434 mmol N, N diethylacrylamide, 129.7-259.4 µmol N, N'-methylbisacrylamide and 87.67-175.34 µmol ammonium peroxodisulfate in 5-10 ml deionized water, and treating for 4-8 hours by using an ultrasonic processor. USE - Method for preparing tissue-induced nanofibrous biomaterial with graphene quantum dot tweezers trapping host cell homing used for controllable sustained-release medical biological material and medical dressing. ADVANTAGE - The prepared tissue-induced nanofibrous biomaterial has biological safety, and can autonomously capture mesenchymal stem cells (MSCs) and homing to the diabetic local tissue, stably release CXCL-8 to recruit MSC homing properties by using sing graphene quantum dots, actively capture cells such as MSCs in tissues or blood vessels, effectively improve MSC homing, promote the vascularization of biomaterials, increase the interaction between biomaterials and the body, and promote tissue damage repair. DETAILED DESCRIPTION - Preparing tissue-induced nanofibrous biomaterial with graphene quantum dot tweezers trapping host cell homing involves putting 5-10 g citric acid powder into a microwave oven, pyrolyzing for 80-90 minutes under 1000 watt power to obtain dark brown liquid, pouring into 10-20 ml of 1 mol/l sodium hydroxide (NaOH) solution, stirring at 4℃ for 30 minutes to obtain a graphene quantum dots (GQDs) solution, dissolving 100-200 mmol CXCL-8, 8.717 µmol -17.434 mmol N,N diethylacrylamide, 129.7-259.4 µmol N,N'-methylbisacrylamide and 87.67-175.34 µmol ammonium peroxodisulfate in 5-10 ml deionized water, treating for 4-8 hours by using an ultrasonic processor, transferring to a round-bottomed flask containing cyclohexane, homogenizing by adding Span80 (RTM:a non-ionic surfactant, based on a natural fatty acid (oleic acid) and sugar alcohol sorbitol) to obtain GQDs/CXCL-8 nanohydrogel solution, stirring continuously the obtained GQDs/CXCL-8 nanohydrogel solution by a magnetic stirrer, adding 50-100 microliter N,N,N',N'-tetramethylethylenediamine, polymerizing under nitrogen for 6-12 hours, washing the resulting precipitated product, centrifuging, placing in a dialysis bag, dialyzing on a magnetic stirrer at room temperature for 3 days to obtain purified GQDs/CXCL-8 nanohydrogel, freezing-drying to obtain GQDs/CXCL-8 nano-hydrogel particle, dissolving 135.0-270.0 mg polycaprolactone and 45.0-90.0 mg collagen in 1 ml hexafluoroisopropanol, stirring magnetically at room temperature for 2-4 hours, adding 30-60 microliter Tween80 (RTM:a nonionic surfactant, polysorbate 80), stirring magnetically for 1-2 hours, adding 2.0-3.0 mg GQDs/CXCL-8 nano-hydrogel particle at a speed of 0.05-0.08 mg/minutes, stirring magnetically for 4-5 hours, dispersing ultrasonically to obtain an electrospinning solution, placing obtained electrospinning solution in a 27-gauge needle syringe, using aluminum foil paper carrying ADM as a receiving carrier, preparing ADM-encapsulated GQDs/CXCL-8 hydrogel-polycaprolactone-collagen nanofiber biomaterial by electrospinning machine subjecting the obtained ADM-encapsulated GQDs/CXCL-8 hydrogel-polycaprolactone-collagen nanofiber biomaterial to vacuum freeze-drying, UV cross-linking, sterilizing to obtain tissue-induced nanofibrous biomaterial with graphene quantum dot tweezers trapping host cell homing and finishing preparation method.