• 专利标题:   Extraction of honey peptide useful for improving cardiovascular and cerebrovascular diseases involves dissolving honey in trichloroacetic acid solution, centrifuging, obtaining honey protein and performing first and second pulse electric treatments.
  • 专利号:   CN115354061-A
  • 发明人:   GAO W
  • 专利权人:   GAO W
  • 国际专利分类:   A23L033/18, A61K038/01, A61P009/00, A61P009/10, C07K001/14, C12N011/14, C12N009/76, C12P021/06
  • 专利详细信息:   CN115354061-A 18 Nov 2022 C12P-021/06 202305 Chinese
  • 申请详细信息:   CN115354061-A CN11019899 24 Aug 2022
  • 优先权号:   CN11019899

▎ 摘  要

NOVELTY - Extraction method of honey peptide for improving cardiovascular and cerebrovascular diseases involves dissolving honey in 2-4 times trichloroacetic acid aqueous solution, storing the solution at -22°C to -18°C for 30-40 minutes, centrifuging the solution at 5000-5200 revolutions per minute (rpm) and 3-6°C for 3-12 minutes, obtaining the precipitate, washing for 2-4 times, placing in 0.1-0.3 times ultra-pure water, freezing the solution at -22°C to -18°C for 10-17 hours to obtain honey protein, adding honey protein into 20-30 times distilled water, uniformly stirring and mixing the solution, adding sodium hydroxide until pH of the solution is 7-7.5, adding 0.1-0.5 times immobilized enzyme filler to the solution, performing first pulse electric treatment to obtain honey polypeptide, and performing second pulse electric treatment of the honey polypeptide. USE - The extraction method of honey peptide is useful for improving cardiovascular and cerebrovascular diseases. The honey peptide extract is useful in medicine and healthcare food (claimed).