• 专利标题:   Preparing composite graphene-encapsulated phospholipase D useful in producing phosphatidylserine, comprises e.g. culturing phospholipase D-producing strain, centrifuging, and mixing crude enzyme solution with sodium bicarbonate solution.
  • 专利号:   CN111304191-A
  • 发明人:   CHEN K, FENG J, WANG X
  • 专利权人:   NANJING KAINUO BIOTECHNOLOGY CO LTD
  • 国际专利分类:   C12N011/14, C12P013/06
  • 专利详细信息:   CN111304191-A 19 Jun 2020 C12N-011/14 202058 Pages: 11 Chinese
  • 申请详细信息:   CN111304191-A CN10120816 26 Feb 2020
  • 优先权号:   CN10120816

▎ 摘  要

NOVELTY - Preparing composite graphene-encapsulated phospholipase D, comprises (i) culturing the phospholipase D-producing strain, centrifuging, and the resulting supernatant is the crude enzyme solution; (ii) after mixing the crude enzyme solution obtained in the step (i) with the sodium bicarbonate solution, adding graphene powder and stirring; and (iii) adding calcium chloride solution to the material obtained in step (ii), stirring, allowing to stand, centrifuging to obtain the product. USE - The composite graphene-encapsulated phospholipase D is useful in producing phosphatidylserine (claimed). ADVANTAGE - The method: avoids the high cost of using graphene as an immobilized carrier by doping low-cost calcium carbonate materials, avoids the disadvantages of too small graphene size, resulting in difficult separation and high technical requirements for production. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included (1) a composite graphene-encapsulated phospholipase D; and (2) a method for producing phosphatidylserine comprising mixing composite graphene-encapsulated phospholipase D, acetate-sodium acetate buffer with L-serine, phosphatidylcholine, and methylene chloride, and reacting.