▎ 摘　　要

NOVELTY - Complex nano-drug delivery system comprises nucleic acid aptamer NH2-AS1411, single-layer graphene oxide (GO), chitooligosaccharides (CO), gamma -polyglutamic acid ( gamma -PGA), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC), N-hydroxysuccinimide (NHS), 2-(N-morpholino)ethanesulfonic acid (MES) buffer and phosphate-buffered saline (PBS) buffer. USE - The complex nano-drug delivery system is useful for loading doxorubicin for preparing cervical cancer drugs and as photothermal agent for tumor ablation (claimed). ADVANTAGE - The complex nano-drug delivery system exhibits synergistic chemotherapy and near-infrared photothermal therapy, hence improves killing efficiency of cancer cells, actively accumulates on tumor site, stimulates release of drugs, and destroys the tumor tissue, is non-toxic and does not cause side effects on the normal tissues and organs of the organism, minimizes damage to non-tumor parts, and can safely and efficiently play the role of tumor treatment in the organism. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: (1) method for preparing the complex nano-drug delivery system, involves (a) dissolving single layer of GO in ultrapure water, ultrasonically pulverizing and centrifuging the ultrasonic solution to remove the unstripped GO to obtain GO suspension, (b) adjusting pH of the GO suspension to 5-6 with MES buffer, adding EDC and NHS in sequence, performing ultrasonic treatment, sealing, reacting in a shaker, centrifuging and discarding the supernatant, (c) dissolving CO in PBS buffer, ultrasonically treating to fully dissolve CO solution, using CO solution to resuspend GO precipitate, adjusting the pH of the mixture to 7.2-7.5 with PBS buffer, performing ultrasonic treatment, sealing, reacting in a shaker, washing by centrifuging and dialyzing to obtain GO-CO, (d) dissolving gamma -PGA in ultrapure water to obtain a gamma -PGA aqueous solution, adjusting the pH of the solution to 5-6 with MES buffer, adding EDC and NHS, performing ultrasonic treatment, sealing and reacting in a shaker to obtain gamma -PGA solution, (e) dissolving GO-CO in ultrapure water, ultrasonically treating to obtain GO-CO solution, adding the activated gamma -PGA solution to the GO-CO solution, adjusting the pH of the mixture to 7.2-7.5 with PBS buffer, performing ultrasonic treatment, sealing, reacting in a shaker, washing the reaction solution by centrifugation, dialyzing and freeze-drying to obtain GO-CO- gamma -PGA powder and (f) dissolving the GO-CO- gamma -PGA powder in 20 mM tris(hydroxymethyl)aminomethane hydrochloride buffer containing 0.1 M potassium chloride, ultrasonically dissolving, adding EDC and NHS in sequence, reacting in a shaker to activate, adding NH2-AS1411 to the reaction solution, then centrifuging, washing and dialyzing to obtain APT-GO-CO- gamma -PGA; and (2) use of the complex nano-drug delivery system for loading doxorubicin in preparation of cervical cancer drugs and as a photothermal agent for tumor ablation, where when the drug delivery system is used for preparing cervical cancer drugs, the required near-infrared laser irradiation intensity is 2 W/cm2 at 808 nm.