▎ 摘　　要

NOVELTY - The preparation of a multi-channel micro-fluidic electrochemical sensing chip involves: forming a graphene oxide film layer on the surface of a base material; coating a zinc salt solution on the surface of the graphene oxide film layer, drying and calcining to obtain a base material combined with a zinc oxide/graphene oxide composite layer; immersing the base material into a mixed solution dissolved with metal salt, polyvinylpyrrolidone, a morphology control agent and meso(4-carboxyphenyl)porphin, drying, and stripping the composite material growing on the base material; preparing a multi-channel electrode comprising a reference electrode (1), a counter electrode (2, 3, 4) and working electrodes (5) on a flexible polymer substrate; preparing ink, coating the ink on the surface of the working electrode, and drying to obtain a multi-channel electrode chip; preparing a microfluidic channel; and placing the multi-channel electrode chip below the micro-fluidic channel. USE - Preparation method of a multi-channel micro-fluidic electrochemical sensing chip used in electrochemical immunosensor (claimed) for detecting biological entity such as blood, urine, saliva. ADVANTAGE - The method integrates the micro-fluidic channel on the surface of the multi-channel electrode chip to accurately control trace samples, thus facilitating the development of various multi-channel and serialized operations and experiments and being more suitable for detecting and researching trace objects to be detected in complex environments. The electrochemical sensing chip array printed in batch can be combined with a microfluidic technology to develop a miniature electrochemical sensor suitable for parallel and combined detection of objects to be detected. DETAILED DESCRIPTION - The preparation method of a multi-channel micro-fluidic electrochemical sensing chip involves: forming a graphene oxide film layer on the surface of a base material; coating a zinc salt solution on the surface of the graphene oxide film layer, drying and calcining to obtain a base material combined with a zinc oxide/graphene oxide composite layer; immersing the base material combined with the zinc oxide/graphene oxide composite layer into a mixed solution dissolved with metal salt, polyvinylpyrrolidone, a morphology control agent and meso(4-carboxyphenyl)porphin, drying after reaction, and stripping the composite material growing on the base material; preparing a multi-channel electrode comprising a reference electrode (1), a counter electrode (5) and working electrodes (2, 3, 4) on a flexible polymer substrate; preparing ink from the composite material, coating the ink on the surface of the working electrode of the multi-channel electrode, and drying to obtain a multi-channel electrode chip; preparing a microfluidic channel according to the multi-channel electrode chip, where the microfluidic channel is provided with a channel for placing a reference electrode, a counter electrode and working electrodes, and a first injection pool (10) respectively communicated with the working electrodes, and a second injection pool (11) communicated with the reference electrode and the counter electrode; and placing the multi-channel electrode chip below the micro-fluidic channel, and packaging. An INDEPENDENT CLAIM is included for the multi-channel micro-fluidic electrochemical sensing chip. DESCRIPTION OF DRAWING(S) - The drawing shows a schematic diagram of the multi-channel micro-fluidic electrochemical sensing chip. 1Reference electrode 2, 3, 4 Working electrodes 5Counter electrode 6Lead 7Circular electrode tip of each electrode 8Electrode lead of each electrode tip 9Contact part of the electrode lead and the lead 10First injection pool 11Second injection pool 12Chamber corresponding to the reference electrode in the microfluidic channel 13Chamber corresponding to the counter electrode in the microfluidic channel 14Chamber corresponding to the working electrode in the microfluidic channel